Graft-infiltrating cells in rats receiving orthotopic semiallogeneic small intestine transplantation with portal or systemic venous drainage

被引:14
作者
Sullivan, B
Cohen, Z
Fu, XM
Levy, G
Plapler, H
Wojcik, D
Gorczynski, RM
机构
[1] UNIV TORONTO,DEPT SURG,TORONTO,ON M5G 2C4,CANADA
[2] UNIV TORONTO,MRC,PROGRAM PROJECT GRP,TORONTO,ON M5G 2C4,CANADA
[3] UNIV TORONTO,DEPT MED,TORONTO,ON M5G 2C4,CANADA
[4] UNIV TORONTO,DEPT IMMUNOL,TORONTO,ON M5G 2C4,CANADA
[5] TORONTO HOSP,TORONTO,ON M5G 2C4,CANADA
关键词
D O I
10.1097/00007890-199609270-00003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The effect of alterations in venous drainage, from either ivc to portal vein (pv), along with peritransplant systemic (ivc) or portal (pv) venous alloimmunization with irradiated semiallogeneic cells, on cell subset recovery in lymphoid organs of Lewis rats re receiving orthotopic small bowel allografts (from Lewis x Brown Norway) F-1, LBNF(1)) was examined, Combined portal, venous drainage and alloimmunization has been reported to increase graft/recipient survival in this model, FAGS analysis using monoclonal antibodies specific for different lymphocyte subsets was performed on cell suspensions of peripheral (P) and mesenteric (M) lymph node (LN), small bowel intraepithelial lymphocytes (SBIEL), and Peyer's patch (PP) lymphocytes on days 2 and 8 posttransplantation, Donor cell contributions to these cellular analyses were estimated by comparison of FAGS staining with polyclonal anti-Lewis or Lewis anti-LBNF(1) antibodies, Control animals received syngeneic grafts, In both syngeneic and semi-allogeneic transplants with pv or ivc drainage there was no consistent difference in cell subsets from in PLN compared with those of control nongrafted rats, Approximately 50% to 60% of these cells were alpha beta TcR(+) with a CD4+/CD8+ ratio of 3-4:1 and a (CD4(+) + CD8(+))/alpha beta TcR(+) ratio of 1:1, Some 5% to 12% ED3(+) cells were also present, In IEL, PIILN, and PP by contrast, there were significant differences in cells recovered from rats with ivc vs, pv drainage of grafts, The most striking changes reflected a decreased CD4+/CD8+ and alpha beta TcR+/gamma delta TcR+ cells in these tissues in rats predestined to show prolongation of allograft survival live vs, pv injected IEL CD4+/CD8+ ratios and alpha beta TcR+/gamma delta TcR+ ratios were 1.0, 0.7 and 5.0, 1.0, respectively, These data are consistent with a proposed role for such gamma delta TcR+ cells in the local regulation of graft rejection.
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收藏
页码:715 / 721
页数:7
相关论文
共 49 条
[1]  
Abbs I C, 1993, Transpl Immunol, V1, P45, DOI 10.1016/0966-3274(93)90058-G
[2]   PROLONGATION OF ALLOGENEIC HEART GRAFT SURVIVAL IN RAT AFTER IMPLANTATION ON PORTAL-VEIN [J].
BOECKX, W ;
SOBIS, H ;
LACQUET, A ;
GRUWEZ, J ;
VANDEPUTTE, M .
TRANSPLANTATION, 1975, 19 (02) :145-149
[3]   PERSISTENCE OF ALLOGENEIC CELLS IN GRAFT AND HOST TISSUES AFTER SMALL-BOWEL TRANSPLANTATION [J].
CLARK, CLI ;
PRICE, BA ;
CRANE, PW ;
LEAR, PA ;
WOOD, RFM .
BRITISH JOURNAL OF SURGERY, 1992, 79 (05) :424-426
[4]  
DALLMAN MJ, 1993, CURR OPIN IMMUNOL, V5, P789
[5]   LYMPHOKINE PRODUCTION BY MITOGEN AND ANTIGEN ACTIVATED MOUSE INTRAEPITHELIAL LYMPHOCYTES [J].
DILLON, SB ;
DALTON, BJ ;
MACDONALD, TT .
CELLULAR IMMUNOLOGY, 1986, 103 (02) :326-338
[6]   UNUSUAL PHENOTYPE OF INTESTINAL INTRAEPITHELIAL LYMPHOCYTES IN THE RAT - PREDOMINANCE OF T-CELL RECEPTOR ALPHA-BETA+ CD2- CELLS AND HIGH EXPRESSION OF THE RT6 ALLOANTIGEN [J].
FANGMANN, J ;
SCHWINZER, R ;
WONIGEIT, K .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (03) :753-760
[7]   IMMUNOREGULATORY FUNCTIONS FOR MURINE INTRAEPITHELIAL LYMPHOCYTES - GAMMA-DELTA T-CELL RECEPTOR POSITIVE (TCR+) T-CELLS ABROGATE ORAL TOLERANCE, WHILE ALPHA-BETA-TCR+ T-CELLS PROVIDE B-CELL HELP [J].
FUJIHASHI, K ;
TAGUCHI, T ;
AICHER, WK ;
MCGHEE, JR ;
BLUESTONE, JA ;
ELDRIDGE, JH ;
KIYONO, H .
JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 175 (03) :695-707
[8]   DYNAMICS OF ALLOSPECIFIC LYMPHOCYTE-T INFILTRATION IN VASCULARIZED HUMAN ALLOGRAFTS [J].
FUNG, JJ ;
ZEEVI, A ;
MARKUS, B ;
ZERBE, TR ;
DUQUESNOY, RJ .
IMMUNOLOGIC RESEARCH, 1986, 5 (02) :149-163
[9]  
GARCIA B, 1990, TRANSPLANT P, V22, P2469
[10]  
GORCZYNSKI RM, 1994, IMMUNOLOGY, V81, P27