A rapid and specific whole blood HPA-1 phenotyping by flow cytometry using two commercialized monoclonal antibodies directed against GP IIIa and GP IIb-IIIa complexes

被引：5

作者：

Sorel, N ^{[1
]}

Brabant, S

Christiaens, L

Brizard, A

Mauco, G

Macchi, L

机构：

[1] Hop Mil, CHU Poitiers, Fac Med & Pharm, Lab Hematol & Malad Sang, F-86021 Poitiers, France

[2] Hop Mil, CHU Poitiers, Fac Med & Pharm, Dept Cardiol, F-86021 Poitiers, France

[3] Hop Mil, CHU Poitiers, Fac Med & Pharm, INSERM ERM324, F-86021 Poitiers, France

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2004年 / 124卷 / 02期

关键词：

flow cytometry; HPA-1; phenotyping; glycoprotein IIIa; P2 monoclonal antibody; SZ21 monoclonal antibody;

D O I：

10.1046/j.1365-2141.2003.04757.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The human platelet antigen 1 (HPA-1) system has been implicated in rare but severe diseases, such as neonatal alloimmune thrombocytopenic purpura, post-transfusion purpura and immune platelet refractoriness. We developed a flow cytometry assay for HPA-1 phenotyping using two commercial monoclonal antibodies, P2 and SZ21, directed against glycoprotein (GP) IIb-IIIa and GP IIIa respectively. One hundred and twenty-seven healthy controls were studied and ratios of mean fluorescence intensity for P2 and SZ21 discriminated between HPA-1a homozygotes and heterozygotes. These two monoclonal antibodies, coupled with flow cytometry represent a rapid and reliable tool for platelet HPA-1 typing to aid diagnosis.

引用

页码：221 / 223

页数：3

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