Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B

被引：174

作者：

Simmen, KA

Singh, J

Luukkonen, BGM

Lopper, M

Bittner, A

Miller, NE

Jackson, MR

Compton, T

Früh, K

机构：

[1] RW Johnson Pharmaceut Res Inst, San Diego, CA 92121 USA

[2] Univ Wisconsin, Mcardle Lab Canc Res, Madison, WI 53706 USA

[3] Battelle Mem Inst, Columbus, OH 43201 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 13期

关键词：

D O I：

10.1073/pnas.121177598

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human cytomegalovirus (HCMV) infection alters the expression of many cellular genes, including IFN-stimulated genes (ISGs) [Zhu. H., Gong, J.-P., Mamtora, G., Gingeras, T. & Shenk, T, (1998) Proc. Natl. Acad. Sci. USA 95, 14470-14475]. By using high-density cDNA microarrays, we show that the HCMV-regulated gene expression profile in fibroblasts does not differ substantially from the response generated by IFN. Furthermore, we identified the specific viral component triggering this response as the envelope glycoprotein B (gB). Cells treated with gB, but not other herpesviral glycoproteins, exhibited the same transcriptional profile as HCMV-infected cells. Thus, the interaction of gB with its as yet unidentified cellular receptor is the principal mechanism by which HCMV alters cellular gene expression early during infection. These findings highlight a pioneering paradigm for the consequences of virus-receptor interactions.

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页码：7140 / 7145

页数：6

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