Pectins and pectolytic enzymes in relation to development and processing of green beans (Phaseolus vulgaris L)

被引：3

作者：

Recourt, K

StolleSmits, T

Laats, JM

Beekhuizen, JG

Ebbelaar, CEM

Voragen, AGJ

Wichers, HJ

vanDijk, C

机构：

[1] Agrotechnological Research Institute (ATO-DLO), Department of Biochemistry and Food Processing, 6700 AA Wageningen

[2] Wageningen Agricultural University, Department of Food Sciences, 6700 EV Wageningen

来源：

PECTINS AND PECTINASES | 1996年 / 14卷

关键词：

D O I：

10.1016/S0921-0423(96)80271-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Processing of green beans involves major changes within the composition of cell wall pectins. About 20% of homogalacturonan is degraded while 65% of rhamnogalacturonan is solubilized. Since beta-eliminative breakdown, which is dependant on the degree of pectin methylesterification, is probably the main mechanism explaining this phenomenon, a biochemical and molecular biological study was initiated on the cell wall enzyme pectin methylesterase [PE]. Two groups of isoenzymes with molecular weights of 33 kDa and 42 kDa and different thermostabilities were partly purified. In addition, it appeared that two PE genes, most likely encoding precursor proteins of 63 kDa, are expressed during pod development.

引用

页码：399 / 404

页数：6

共 10 条

[1]

De Vries J.A., Rombouts F.M., Voragen A.G.J., Pilnik W., Carbohydr. Polym., 2, pp. 25-33, (1982)

[2]

Tucker G.A., Mitchell J., Biosynthesis and manipulation of plant products, pp. 55-103, (1993)

[3]

Bartolome L.G., Hoff J.E., J. Agric. Food Chem., 20, pp. 266-270, (1972)

[4]

Ryden P., Selvendran R.R., Carbohydr. Res., 195, pp. 257-272, (1989)

[5]

Hall L.N., Bird C.R., Picton S., Tucker G.A., Seymour G.B., Grierson D., Plant Mol. Biol., 25, pp. 313-318, (1994)

[6]

Albani D., Altosaar I., Arnison P.G., Fabijanski S.F., Plant Mol. Biol., 16, pp. 501-513, (1991)

[7]

Recourt K., Laats J.M., Stolle-Smits T., Wichers H.J., van Dijk C., Ebbelaar C.E.M., GenBank Data Base, (1995)

[8]

von Heijne G., J. Mol. Biol., 189, pp. 239-242, (1986)

[9]

Richard L., Qin L.-X., Goldberg R., FEBS Letters, 355, pp. 135-139, (1994)

[10]

Stolle-Smits T., Beekhuizen J.G., van Dijk C., Voragen A.G.J., Recourt K., J. Agric. and Food Chem., 43, pp. 2480-2486, (1995)

← 1 →