Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts

被引:16
作者
Chen, Chanchan [1 ]
Zhan, Jianying [2 ]
Ling, Junqi [3 ]
Du, Yu [3 ]
Hou, Yuluan [3 ]
机构
[1] Shenzhen Childrens Hosp, Dept Stomatol, Shenzhen 518038, Guangdong, Peoples R China
[2] Cent S Univ, Dept Operat Dent & Endodont, Xiangya Sch Stomatol, Xiangya Stomatol Hosp, Changsha 410083, Hunan, Peoples R China
[3] Sun Yat Sen Univ, Dept Operat Dent & Endodont, Guanghua Sch Stomatol, Res Inst Stomatol,Guangdong Prov Key Lab Stomatol, 56 Ling Yuan Xi Rd, Guangzhou 510055, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
naked cuticle homolog 2; dental follicle stem; progenitor cells; differentiation; osteoblasts; Wnt; -catenin; GENE-EXPRESSION; BETA-CATENIN; OSTEOGENIC DIFFERENTIATION; PLASMA-MEMBRANE; IN-VITRO; WNT; PATHWAY; GROWTH; METASTASIS; ANTAGONIZE;
D O I
10.3892/ijmm.2018.3822
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
100103 [病原生物学]; 100218 [急诊医学];
摘要
Dental follicle stem/progenitor cells have the potential to undergo osteogenesis. naked cuticle homolog 2 (Nkd2) is a signal-inducible feedback antagonist of the canonical Wnt signaling pathway. The purpose of the present study was to investigate the function of Nkd2 in the differentiation of dental follicle stem/progenitor cells (DFSCs) into osteoblasts. Immunohistochemistry, reverse transcription-quantitative polymerase chain reaction and western blotting were employed to detect Nkd2 expression in rat DFSCs. In addition, rat DFSCs (rDFSCs) were transfected with small interfering RNAs to examine the effect of Nkd2 on the differentiation of these cells into osteoblasts. Furthermore, the function of Nkd2 in the Wnt/-catenin pathway in rDFSCs was investigated using -catenin/T-cell factor luciferase activity assays and western blotting. It was revealed that the expression of Nkd2 was upregulated during the differentiation of rDFSCs into osteoblasts. Furthermore, osteoblast differentiation ability and Wnt/-catenin pathway activity were significantly decreased in Nkd2-silenced rDFSCs compared with the si-NC group (P<0.05 and P<0.001, respectively). The results suggest that Nkd2 promotes the differentiation of rDFSCs into osteoblasts through Wnt/-catenin signaling.
引用
收藏
页码:2403 / 2414
页数:12
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