Separation and identification of active components in the extract of Rheum natural products by microemulsion electrokinetic chromatography

被引：54

作者：

Li, GB ^{[1
]}

Chen, XG ^{[1
]}

Liu, MC ^{[1
]}

Hu, ZD ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

来源：

ANALYST | 1998年 / 123卷 / 07期

关键词：

microemulsion electrokinetic chromatography; capillary electrophoresis; active component; Rheum plant;

D O I：

10.1039/a800353j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new microemulsion electrokinetic chromatographic method based on a butan-1-ol-ethyl acetate-mater microemulsion for the separation and determination of five components in Rheum plant was developed. The migration behavior of these analytes was studied and the separation efficiency was optimized by the systematic examination of the microemulsion content and acetonitrile concentration in the run buffer. Resolution was enhanced for neutral compounds by adjusting the microemulsion and acetonitrile content. Extracts of a Rheum plant sample were analyzed and five active components were determined. An equation describing the migration behavior of compounds at different acetonitrile concentrations was also derived by simulation.

引用

页码：1501 / 1505

页数：5

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