Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system

被引：32

作者：

Avlami, Athina ^{[2
]}

Bekris, S. ^{[2
]}

Ganteris, G. ^{[3
]}

Kraniotaki, Eleni ^{[4
]}

Malamou-Lada, Eleni ^{[3
]}

Orfanidou, Maria ^{[3
]}

Paniara, Olga ^{[4
]}

Pantazatou, Angeliki ^{[2
]}

Papagiannitsis, C. C. ^{[1
]}

Platsouka, Evangelia ^{[4
]}

Stefanou, Ioanna ^{[2
]}

Tzelepi, Eva ^{[1
]}

Vagiakou, Helen ^{[3
]}

Miriagou, Vivi ^{[1
]}

机构：

[1] Hellenic Pasteur Inst, Bacteriol Lab, Athens 11521, Greece

[2] Laikon Gen Hosp, Dept Microbiol, Athens, Greece

[3] Gen Hosp Athens G Gennimatas, Dept Microbiol, Athens, Greece

[4] Evangelismos Gen Hosp, Dept Microbiol, Athens, Greece

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2010年 / 83卷 / 02期

关键词：

Metallo-beta-lactamase; bla(VIM); bla(IMP); Multiplex PCR; KLEBSIELLA-PNEUMONIAE; GREEK HOSPITALS; CARBAPENEMASES; PATHOGENS; STRAINS; ISSUES; VIM-1;

D O I：

10.1016/j.mimet.2010.08.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

hyplex (R)-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-beta-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential ;diagnostic utility. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：185 / 187

页数：3

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