Rapid detection of mutations in the 23S rRNA gene of Helicobacter pylori that confers resistance to clarithromycin treatment to the bacterium

被引：52

作者：

Matsumura, M

Hikiba, Y

Ogura, K

Togo, G

Tsukuda, I

Ushikawa, K

Shiratori, Y

Omata, M

机构：

[1] Asahi Life Fdn, Inst Adult Dis, Shinjuku Ku, Tokyo 1600023, Japan

[2] Univ Tokyo, Fac Med, Dept Internal Med, Div Gastroenterol, Tokyo 1138655, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 02期

关键词：

D O I：

10.1128/JCM.39.2.691-695.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a new method capable of detecting point mutations in the 23S rRNA gene of Helicobacter pylori using a LightCycler. Our method can detect a mutation in this gene in less than 1 h and can process many samples at once, thereby contributing to the selection of patients suitable for clarithromycin-based therapy.

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收藏

页码：691 / 695

页数：5

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