Electron transfer between an electrochemically deposited glucose oxidase Cu[II] complex and an electrode

被引：12

作者：

Haruyama, T ^{[1
]}

Aizawa, M ^{[1
]}

机构：

[1] Tokyo Inst Technol, Fac Biosci & Biotechnol, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 09期

关键词：

glucose oxidase; Cu[II; glucose oxidase Cu[II] complex; electron transfer; electrochemical deposition; molecular interface;

D O I：

10.1016/S0956-5663(98)00029-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Glucose oxidase (GOD) complexed with Cu[II] was deposited on the electrode surface with retention of enzyme activity by an electrochemical reduction of Cu[II]. The deposited GOD showed an apparently reversible electron transfer to an electrode through the coordinated Cu. The GOD/Cu electrode responded to glucose in anodic current due to an electrochemical oxidation of enzymatically generated FADH. In conclusion, the electron transfer was mediated by bound Cu which was situated at an active center of GOD. The GOD/Cu system provided an "atomic interface" to perform a smooth electron transfer between a flavoenzyme and an electrode. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：1015 / 1022

页数：8

共 22 条

[1] PREPARATION OF THIN PROTEIN MEMBRANES BY THE ELECTROCHEMICALLY CONTROLLED ADSORPTION AND ITS APPLICATION [J].