Globin and globin gene structure of the nerve myoglobin of Aphrodite aculeata

被引:51
作者
Dewilde, S
Blaxter, M
VanHauwaert, ML
Vanfleteren, J
Esmans, EL
Marden, M
Griffon, N
Moens, L
机构
[1] UNIV ANTWERP,DEPT BIOCHEM,B-2610 ANTWERP,BELGIUM
[2] UNIV EDINBURGH,INST CELL ANIM & POPULAT BIOL,ASHWORTH LABS,EDINBURGH EH9 3JT,MIDLOTHIAN,SCOTLAND
[3] STATE UNIV GHENT,DEPT MORPHOL SYSTEMAT & ECOL,B-9000 GHENT,BELGIUM
[4] UNIV ANTWERP,RIJKSUNIV CTR ANTWERP,NUCLEOSIDE RES & MASS SPECTROMETRY UNIT,B-2020 ANTWERP,BELGIUM
[5] HOP BICETRE,INSERM,U299,F-94275 LE KREMLIN BICETR,FRANCE
关键词
D O I
10.1074/jbc.271.33.19865
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The globin of the nerve cord of the polychaete annelid Aphrodite aculeata was isolated and purified to homogeneity, The native molecule has a pi of 6.3 and acts as a dimer of two identical M(r) 15,644.5 polypeptide chains as determined by electrospray mass spectrometry. It has an average affinity for oxygen (P-50 = 1.24 torr) resulting from fast association (k(on) = 170 x 10(6) M(-1). s(-1)) and dissociation rates (k(off) = 360 s(-1)). The partial primary structure of this nerve globin was determined at the protein level and completed and confirmed by translation of the cDNA sequence. The globin chain has 150 amino acid residues and a calculated M(r) of 15,602.69 strongly suggesting that the amino terminus is acetylated. The absence of a leader sequence and the lack of Cys at the positions NA2 and H9 needed for the formation of the high M(r) complexes found in extracellular annelid globins classify the Aphrodite globin with the cellular globin species. The Aphrodite nerve globin is unlikely to represent a separate globin family, as cDNA derived primers detect globin messenger RNA in muscle, gut, and pharynx tissue as well. The gene encoding this globin species is interrupted by a single intron, inserted at position G7.0. Comparison to other globin gene structures strongly suggest that introns can be lost independently, rather than simultaneously as a result of a single conversion event as suggested previously (Lewin, R. (1984) Science 226, 328).
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页码:19865 / 19870
页数:6
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