Structure of CBM4 from Clostridium thermocellum cellulase K

被引：8

作者：

Alahuhta, Markus ^{[1
]}

Luo, Yonghua ^{[1
]}

Ding, Shi-You ^{[1
]}

Himmel, Michael E. ^{[1
]}

Lunin, Vladimir V. ^{[1
]}

机构：

[1] Natl Renewable Energy Lab, BioSci Ctr, Golden, CO 80401 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2011年 / 67卷

关键词：

CARBOHYDRATE-BINDING MODULES;

D O I：

10.1107/S1744309111003307

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, a 2.0 angstrom resolution X-ray structure of Clostridium thermocellum cellulase K family 4 carbohydrate-binding module (CelK CBM4) is reported. The resulting structure was refined to an R factor of 0.212 and an R-free of 0.274. Structural analysis shows that this new structure is very similar to the previously solved structure of C. thermocellum CbhA CBM4. Most importantly, these data support the previously proposed notion of an extended binding pocket using a novel tryptophan-containing loop that may be highly conserved in clostridial CBM4 proteins.

引用

页码：527 / 530

页数：4

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