EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD

被引：18

作者：

Ji, AM ^{[1
]}

Dao, D ^{[1
]}

Chen, JX ^{[1
]}

MacLellan, WR ^{[1
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Cardiovasc Res Labs, Los Angeles, CA 90095 USA

来源：

GENE | 2003年 / 318卷

关键词：

cloning; p300; differentiation; skeletal muscle; MyoD;

D O I：

10.1016/j.gene.2003.06.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Skeletal muscle differentiation has been shown to be dependent on the expression of Rb and p300. We recently cloned a novel inhibitor of muscle differentiation called EID-1, which interacted with both of these factors. In a database search for related molecules, we have cloned and characterized a new EID-1 family member, EID-2. This 28-kDa protein encodes a 236-amino-acid protein with significant similarity to EID-1 in its C-terminus. EID-2 displays developmentally regulated expression with high-levels in adult heart and brain. Overexpression of EID-2 inhibits muscle-specific gene expression through inhibition of MyoD-dependent. transcription. This inhibitory effect on gene expression can be explained by EID-2's ability to associate with and inhibit the acetyltransferase activity of p300. These data suggest that EID-1 and -2 represent a novel family of proteins that negatively regulate differentiation through a p300-dependent mechanism. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：35 / 43

页数：9

共 27 条

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