Interleukin-1 beta (IL-1 beta) is one of the main cytokines involved in the inflammatory response; it has multiple effects that can contribute to cell damage, one of which is the upregulation of the inducible form of nitric oxide (NO) synthase (NOS2) in certain cell types. We demonstrated previously that in vivo, cortical microglial inflammatory responses were increased when noradrenaline (NE) levels were depleted, suggesting that NE can reduce microglial activation. In the present report, we examined the role of IL-1 beta in neurotoxicity induced by microglial-conclitioned media, and possible neuroprotective effects of NE. Incubation of cortical neurons with conditioned media (CM) obtained from lipopolysaccharide (LPS)-treated microglia induced neuronal NOS2 expression and increased neuronal cell death, and these responses were reduced if the neurons were coincubated with interleukin-1 receptor antagonist. Cotreatment of microglial cells with LIPS plus NE potently blocked IL-1 beta production and reduced the ability of the CM to induce neuronal NOS2 and cell death. These results suggest that microglial release of IL-1 beta is an important activator of neuronal inflammatory responses, and that protective effects of NE upon neurons involve a reduction of microglial-derived IL-1 beta. (c) 2005 wiley-Liss, Inc.
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Ist Super Sanita, Lab Organ & Syst Pathophysiol, Neurophysiol Unit, I-00161 Rome, ItalyIst Super Sanita, Lab Organ & Syst Pathophysiol, Neurophysiol Unit, I-00161 Rome, Italy
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Univ Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
West Side Vet Affairs Res Div, Chicago, IL USA
Catholic Univ, Sch Med, Inst Pharmacol, Rome, ItalyUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
Dello Russo, Cinzia
Boullerne, Anne I.
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Univ Chicago, Dept Neurol, Chicago, IL 60637 USAUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
Boullerne, Anne I.
Gavrilyuk, Vitaliy
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Univ Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
West Side Vet Affairs Res Div, Chicago, IL USAUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
Gavrilyuk, Vitaliy
Feinstein, Douglas L.
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Univ Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
West Side Vet Affairs Res Div, Chicago, IL USAUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
机构:
Ist Super Sanita, Lab Organ & Syst Pathophysiol, Neurophysiol Unit, I-00161 Rome, ItalyIst Super Sanita, Lab Organ & Syst Pathophysiol, Neurophysiol Unit, I-00161 Rome, Italy
机构:
Univ Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
West Side Vet Affairs Res Div, Chicago, IL USA
Catholic Univ, Sch Med, Inst Pharmacol, Rome, ItalyUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
Dello Russo, Cinzia
Boullerne, Anne I.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Chicago, Dept Neurol, Chicago, IL 60637 USAUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
Boullerne, Anne I.
Gavrilyuk, Vitaliy
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h-index: 0
机构:
Univ Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
West Side Vet Affairs Res Div, Chicago, IL USAUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
Gavrilyuk, Vitaliy
Feinstein, Douglas L.
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h-index: 0
机构:
Univ Illinois, Dept Anesthesiol, Chicago, IL 60607 USA
West Side Vet Affairs Res Div, Chicago, IL USAUniv Illinois, Dept Anesthesiol, Chicago, IL 60607 USA