Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue

被引：46

作者：

Kim, Hwan Myting ^{[1
]}

Kim, Bo Ra ^{[1
]}

An, Myoung Jin ^{[1
]}

Hong, Jin Hee ^{[2
]}

Lee, Kyoung J. ^{[2
]}

Cho, Bong Rae ^{[1
]}

机构：

[1] Korea Univ, Dept Chem, Mol Optoelect Lab, Seoul 136701, South Korea

[2] Korea Univ, Natl Creat Res Initiat Ctr Neurodynam, Seoul 136701, South Korea

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2008年 / 14卷 / 07期

关键词：

calcium; fluorescent probes; imaging agents; live tissue; two-photon microscopy;

D O I：

10.1002/chem.200701453

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

2-Acetyl-6-(dimethylamino)naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 mu m in live tissues for 1100-4000s using two-photon microscopy with no artifacts of photobleaching.

引用

页码：2075 / 2083

页数：9

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