The structure of the O-specific polysaccharide of the lipopolysaccharide from Pantoea agglomerans strain FL1

被引:13
作者
Cimmino, Alessio [1 ,2 ]
Marchi, Guido [3 ]
Surico, Giuseppe [3 ]
Hanuszkiewicz, Anna [1 ]
Evidente, Antonio [2 ]
Holst, Otto [1 ]
机构
[1] Leibniz Ctr Med & Biosci, Res Ctr Borstel, Div Struct Biochem, D-23845 Borstel, Germany
[2] Univ Naples Federico 2, Dipartimento Sci Suolo Pianta Ambiente & Prod Ani, I-80055 Portici, NA, Italy
[3] Univ Florence, Dipartimento Biotecnol Agrarie Patol Vegetale, I-50144 Florence, Italy
关键词
olive knot; Pantoea agglomerans; lipopolysaccharide; O-specific polysaccharide; NMR spectroscopy;
D O I
10.1016/j.carres.2007.10.023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A neutral O-specific polysaccharide consisting of D-rhamnose was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant pathogenic bacterium Pantoea agglomerans strain FL1, a common epiphyte of many plant species, and associated with Pseudomonas savastanoi pv. savastanoi in young and apparently intact olive knots. By means of compositional and methylation analyses, and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear tetrasaccharide of the structure: -> 2)-alpha-D-Rhap-(1 -> 2)-beta-D-Rhap-(1 -> 3)-alpha-D-Rhap-(1 -> 2)-alpha-D-rhap-(1 -> (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:392 / 396
页数:5
相关论文
共 23 条
[1]  
[Anonymous], BIOACTIVE NATURAL PR
[2]  
Bock K., 1973, TETRAHEDRON LETT, V14, P1037
[3]   A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].
CIUCANU, I ;
KEREK, F .
CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217
[4]  
Corsaro M. M., 2001, Recent Research Developments in Phytochemistry, V5, P119
[5]  
Down J.M., 2000, ANNU REV PHYTOPATHOL, V38, P241
[6]  
ERCOLANI G. L., 1971, Phytopathologia Mediterranea, V10, P130
[7]  
GERWIG GJ, 1979, CARBOHYD RES, V77, P1, DOI 10.1016/S0008-6215(00)83788-X
[8]   COMPUTER-ASSISTED STRUCTURAL-ANALYSIS OF POLYSACCHARIDES WITH AN EXTENDED VERSION OF CASPER USING H-1-NMR AND C-13-NMR DATA [J].
JANSSON, PE ;
KENNE, L ;
WIDMALM, G .
CARBOHYDRATE RESEARCH, 1989, 188 :169-191
[9]  
Jansson PE, 1999, ENDOTOXIN IN HEALTH AND DISEASE, P155
[10]   Investigation of the chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharide [J].
Kaczynski, Zbigniew ;
Braun, Sebastian ;
Lindner, Buko ;
Niehaus, Karsten ;
Holst, Otto .
JOURNAL OF ENDOTOXIN RESEARCH, 2007, 13 (02) :101-108