Synthesis, characterization and cytocompatibility of polyurethaneurea elastomers with designed elastase sensitivity

被引:97
作者
Guan, JJ
Wagner, WR
机构
[1] Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA
[2] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15261 USA
[3] Univ Pittsburgh, Dept Chem Engn, Pittsburgh, PA 15261 USA
关键词
D O I
10.1021/bm0503322
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In designing a synthetic scaffold for engineering soft, mechanically active tissues, desirable properties include elasticity, support of cell adhesion and growth, ease of processability, and responsiveness to in vivo remodeling. To achieve these properties, we have developed a family of thermoplastic elastomers, polyurethaneureas (PUs), that possess enzymatic remodeling capabilities in addition to simple hydrolytic lability. PUs were synthesized using either polycaprolactone or triblock copolymer polycaprolactone-b-poly(ethylene glycol)-b-polycaprolactone as the soft segment, 1,4-butanediisocyanate as the hard segment, and the peptide Ala-Ala-Lys as a chain extender. The synthesized PUs had high molecular weights, low glass transition temperatures (<-54 degrees C), and were flexible with breaking strains of 670-890% and tensile strengths of 15-28 MPa. Incubation in buffered saline without elastase for 8 weeks resulted in mass loss from 12% to 18% depending on soft segment composition. The degradation significantly increased (p < 0.05) in the presence of elastase, ranging from 19% to 34% with degradation products showing no cytotoxicity. To encourage cell adhesion, PUs were surface-modified with radio frequency glow discharge followed by coupling of Arg-Gly-Asp-Ser (RGDS). Endothelial cell adhesion was > 140% of tissue culture polystyrene on PU surfaces and >200% on RGDS-modified surfaces. The synthesized PUs thus combine mechanical, chemical, and bioresponsive properties that might be employed in soft-tissue engineering applications.
引用
收藏
页码:2833 / 2842
页数:10
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