Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing

被引：799

作者：

Cusanovich, Darren A. ^{[1
]}

Daza, Riza ^{[1
]}

Adey, Andrew ^{[2
]}

Pliner, Hannah A. ^{[1
]}

Christiansen, Lena ^{[3
]}

Gunderson, Kevin L. ^{[3
]}

Steemers, Frank J. ^{[3
]}

Trapnell, Cole ^{[1
]}

Shendure, Jay ^{[1
]}

机构：

[1] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA

[2] Oregon Hlth & Sci Univ, Dept Mol & Med Genet, Portland, OR 97201 USA

[3] Illumina Inc, Adv Res Grp, San Diego, CA USA

来源：

SCIENCE | 2015年 / 348卷 / 6237期

关键词：

RNA-SEQ; HUMAN GENOME; IN-VITRO; TRANSPOSITION; CONTIGUITY; EXPRESSION; FATE;

D O I：

10.1126/science.aab1601

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Technical advances have enabled the collection of genome and transcriptome data sets with single-cell resolution. However, single-cell characterization of the epigenome has remained challenging. Furthermore, because cells must be physically separated before biochemical processing, conventional single-cell preparatory methods scale linearly. We applied combinatorial cellular indexing to measure chromatin accessibility in thousands of single cells per assay, circumventing the need for compartmentalization of individual cells. We report chromatin accessibility profiles from more than 15,000 single cells and use these data to cluster cells on the basis of chromatin accessibility landscapes. We identify modules of coordinately regulated chromatin accessibility at the level of single cells both between and within cell types, with a scalable method that may accelerate progress toward a human cell atlas.

引用

页码：910 / 914

页数：5

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