Solution structure of a human minimembrane protein Ost4, a subunit of the oligosaccharyltransferase complex

被引：21

作者：

Gayen, Shovanlal ^{[1
]}

Kang, CongBao ^{[1
]}

机构：

[1] Agcy Sci Technol & Res, Ctr Expt Therapeut, Singapore 138669, Singapore

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 409卷 / 03期

关键词：

NMR; Membrane protein; Oligosaccharyltransferase; Ost4; MEMBRANE-PROTEIN; NMR; POLYPEPTIDE;

D O I：

10.1016/j.bbrc.2011.05.050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligosaccharyltransferase COST) is a membrane associated enzyme complex that mediates transfer of an oligosaccharide onto asparagine residue of a protein. Human Ost4 is a small membrane protein and belongs to one of the seven subunits of human OST. This study determined the solution structure of human Ost4 in solvent system using NMR spectroscopy. Ost4 was demonstrated that the residues 5-30 adopt an alpha-helical structure. A kink structure was observed in the transmembrane domain, which may be important for its function. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：572 / 576

页数：5

共 21 条

[1] The OST4 gene of Saccharomyces cerevisiae encodes an unusually small protein required for normal levels of oligosaccharyltransferase activity [J].

Chi, JH ;

Roos, J ;

Dean, N .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (06) :3132-3140

[2] NMR structure of the integral membrane protein OmpX [J].

Fernández, C ;

Hilty, C ;

Wider, G ;

Güntert, P ;

Wüthrich, K .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 336 (05) :1211-1221

[3] Solution structure of the transmembrane H+-transporting subunit c of the F1F0 ATP synthase [J].

Girvin, ME ;

Rastogi, VK ;

Abildgaard, F ;

Markley, JL ;

Fillingame, RH .

BIOCHEMISTRY, 1998, 37 (25) :8817-8824

[4]

Guntert Peter, 2004, Methods Mol Biol, V278, P353

[5] Structure of KCNE1 and implications for how it modulates the KCNQ1 potassium channel [J].

Kang, Congbao ;

Tian, Changlin ;

Soennichsen, Frank D. ;

Smith, Jarrod A. ;

Meiler, Jens ;

George, Alfred L., Jr. ;

Vanoye, Carlos G. ;

Kim, Hak Jun ;

Sanders, Charles R. .

BIOCHEMISTRY, 2008, 47 (31) :7999-8006

[6] An evolving view of the eukaryotic oligosaccharyltransferase [J].

Kelleher, DJ ;

Gilmore, R .

GLYCOBIOLOGY, 2006, 16 (04) :47R-62R

[7] The oligosaccharyltransferase complex from yeast [J].

Knauer, R ;

Lehle, L .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1999, 1426 (02) :259-273

[8] Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes [J].

Krogh, A ;

Larsson, B ;

von Heijne, G ;

Sonnhammer, ELL .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 305 (03) :567-580

[9] Spatial structure of the M2 transmembrane segment of the nicotinic acetylcholine receptor α-subunit [J].

Pashkov, VS ;

Maslennikov, IV ;

Tchikin, LD ;

Efremov, RG ;

Ivanov, VT ;

Arseniev, AS .

FEBS LETTERS, 1999, 457 (01) :117-121

[10] Strategies for dealing with conformational sampling in structural calculations of flexible or kinked transmembrane peptides [J].

Rainey, Jan K. ;

Fliegel, Larry ;

Sykes, Brian D. .

BIOCHEMISTRY AND CELL BIOLOGY, 2006, 84 (06) :918-929

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