A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA

被引：85

作者：

Goranov, AI ^{[1
]}

Katz, L ^{[1
]}

Breier, AM ^{[1
]}

Burge, CB ^{[1
]}

Grossman, AD ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 36期

关键词：

DNA replication; transcription; DNA microarrays; Bacillus subtilis;

D O I：

10.1073/pnas.0506174102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Organisms respond to perturbations in DNA replication. We characterized the global transcriptional response to inhibition of DNA replication in Bacillus subtilis. We focused on changes that were independent of the known recA-dependent global DNA damage (SOS) response. We found that overlapping sets of genes are affected by perturbations in replication elongation or initiation and that this transcriptional response serves to inhibit cell division and maintain cell viability. Approximately 20 of the operons (> 50 genes) affected have potential DnaA-binding sites and are probably regulated directly by DnaA, the highly conserved replication initiation protein and transcription factor. Many of these genes have homologues and recognizable DnaA-binding sites in other bacteria, indicating that a DnaA-mediated response, elicited by changes in DNA replication status, may be conserved.

引用

页码：12932 / 12937

页数：6

共 66 条

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