Oxidative preconditioning affords protection against carbon tetrachloride-induced glycogen depletion and oxidative stress in rats

被引：52

作者：

Candelario-Jalil, E ^{[1
]}

Mohammed-Al-Dalain, S ^{[1
]}

Fernández, OSL ^{[1
]}

Menéndez, S ^{[1
]}

Pérez-Davison, G ^{[1
]}

Merino, N ^{[1
]}

Sam, S ^{[1
]}

Ajamieh, HH ^{[1
]}

机构：

[1] Univ Havana, Ctr Res & Viol Evaluat, Inst Pharm & Food Sci, Havana 10600, Cuba

来源：

JOURNAL OF APPLIED TOXICOLOGY | 2001年 / 21卷 / 04期

关键词：

ozone; oxidative preconditioning; carbon tetrachloride hepatotoxicity; oxidative stress; reactive oxygen species; metabolic disorders; glycogen depletion; anaerobic glycolysis;

D O I：

10.1002/jat.758

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The rectal insufflation of a judicious dose of ozone, selected from that used in clinical practice, is able to promote oxidative preconditioning or oxidative stress tolerance preventing the hepatocellular damage mediated by free radicals. In order to evaluate the effects of ozone oxidative preconditioning on carbon tetrachloride-mediated hepatotoxicity, the following experimental protocol was designed: group 1 (negative control, sunflower oil i.p.); group 2 (CCl4 in sunflower oil, 1 ml kg(-1) i.p.); group 3 (15 ozone-oxygen pretreatments at a dose of 1 mg kg(-1) via rectal insufflation + CCl4 as in group 2); group 4 (ozone control group, 15 ozone-oxygen pretreatments + sunflower oil i.p.). Ozone pretreatment prevented glycogen depletion (as demonstrated by biochemical and histopathological findings) and avoided lactate overproduction associated with the hepatotoxic effects Of CCl4. The administration Of CCl4 increased lipid peroxidation (as measured by thiobarbituric acid-reactive substances) and uric acid levels and inhibited superoxide dismutase activity. All these deleterious effects induced by CCl4 were prevented by ozone pretreatment. The administration of ozone without CCl4 (ozone control group) did not produce any changes in the evaluated parameters. Our results showed that ozone treatment, in our experimental conditions, was able to prevent anaerobic glycolysis and oxidative stress induced by CCl4. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：297 / 301

页数：5

共 36 条

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