Structure-function relationships of β-D-glucan endo- and exohydrolases from higher plants

被引:105
作者
Hrmova, M [1 ]
Fincher, GB [1 ]
机构
[1] Univ Adelaide, Dept Plant Sci, Glen Osmond, SA 5064, Australia
基金
澳大利亚研究理事会;
关键词
catalytic mechanism; cell wall hydrolysis; monocotyledons; protein modelling; substrate binding; subsite mapping;
D O I
10.1023/A:1010619128894
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
(1 -->3),(1 -->4)-beta -D-Glucans represent an important component of cell walls in the Poaceae family of higher plants. A number of glycoside endo- and exohydrolases is required for the depolymerization of (1 -->3),(1 -->4)-beta -D-glucans in germinated grain or for the partial hydrolysis of the polysaccharide in elongating vegetative tissues. The enzymes include (1 -->3),(1 -->4)-beta -D-glucan endohydrolases (EC 3.2.1.73), which are classified as family 17 glycoside hydrolases, (1 -->4)-beta -D-glucan glucohydrolases (family 1) and beta -D-glucan exohydrolases (family 3). Kinetic analyses of hydrolytic reactions enable the definition of action patterns, the thermodynamics of substrate binding, and the construction of subsite maps. Mechanism-based inhibitors and substrate analogues have been used to study the spatial orientation of the substrate in the active sites of the enzymes, at the atomic level. The inhibitors and substrate analogues also allow us to define the catalytic mechanisms of the enzymes and to identify catalytic amino acid residues. Three-dimensional structures of (1 -->3),(1 -->4)-beta -D-glucan endohydrolases, (1 -->4)-beta -D-glucan glucohydrolases and beta -D-glucan exohydrolases are available or can be reliably modelled from the crystal structures of related enzymes. Substrate analogues have been diffused into crystals for solving of the three-dimensional structures of enzyme-substrate complexes. This information provides valuable insights into potential biological roles of the enzymes in the degradation of the barley (1 -->3),(1 -->4)-beta -D-glucans during endosperm mobilization and in cell elongation.
引用
收藏
页码:73 / 91
页数:19
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