Saturated Reconstruction of a Volume of Neocortex

被引：681

作者：

Kasthuri, Narayanan ^{[1
,2
]}

Hayworth, Kenneth Jeffrey ^{[1
,2
]}

Berger, Daniel Raimund ^{[1
,2
]}

Schalek, Richard Lee ^{[1
,2
]}

Conchello, Jose Angel ^{[1
,2
]}

Knowles-Barley, Seymour ^{[1
,2
]}

Lee, Dongil ^{[1
,2
]}

Vazquez-Reina, Amelio ^{[3
]}

Kaynig, Verena ^{[3
]}

Jones, Thouis Raymond ^{[1
,2
,3
]}

Roberts, Mike ^{[3
]}

Morgan, Josh Lyskowski ^{[1
,2
]}

Tapia, Juan Carlos ^{[1
,2
]}

Seung, H. Sebastian ^{[7
]}

Roncal, William Gray ^{[4
]}

Vogelstein, Joshua Tzvi ^{[8
]}

Burns, Randal ^{[4
]}

Sussman, Daniel Lewis ^{[5
]}

Priebe, Carey Eldin ^{[6
]}

Pfister, Hanspeter ^{[3
]}

Lichtman, Jeff William ^{[1
,2
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA

[3] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[4] Johns Hopkins Univ, Dept Comp Sci, Baltimore, MD 21218 USA

[5] Harvard Univ, Dept Stat, Cambridge, MA 02138 USA

[6] Johns Hopkins Univ, Dept Appl Math & Stat, Baltimore, MD 21218 USA

[7] MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA

[8] Duke Univ, Dept Stat Sci & Neurobiol, Durham, NC 27708 USA

来源：

CELL | 2015年 / 162卷 / 03期

基金：

美国国家科学基金会;

关键词：

PYRAMIDAL NEURONS; DIRECTION-SELECTIVITY; SYNAPTIC CONNECTIONS; WIRING SPECIFICITY; DENDRITIC SPINES; RAT HIPPOCAMPUS; TOTAL NUMBER; SYNAPSES; CIRCUIT; CORTEX;

D O I：

10.1016/j.cell.2015.06.054

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe automated technologies to probe the structure of neural tissue at nanometer resolution and use them to generate a saturated reconstruction of a sub-volume of mouse neocortex in which all cellular objects (axons, dendrites, and glia) and many sub-cellular components (synapses, synaptic vesicles, spines, spine apparati, postsynaptic densities, and mitochondria) are rendered and itemized in a database. We explore these data to study physical properties of brain tissue. For example, by tracing the trajectories of all excitatory axons and noting their juxtapositions, both synaptic and nonsynaptic, with every dendritic spine we refute the idea that physical proximity is sufficient to predict synaptic connectivity (the so-called Peters' rule). This online minable database provides general access to the intrinsic complexity of the neocortex and enables further data-driven inquiries.

引用

页码：648 / 661

页数：14

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