Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients

被引：16

作者：

Binder, T

Siegert, W

Kruse, A

Oettle, H

Wilborn, F

Peng, RQ

Timm, H

Neuhaus, P

Schmidt, CA

机构：

[1] Humboldt Univ, Klin Innere Med MS Hamatol & Onkol, D-13353 Berlin, Germany

[2] Humboldt Univ, Klin Allgemein Visceral & Transplantat Chirurg, D-13353 Berlin, Germany

[3] Univ Jena Klinikum, Inst Transfus Med, Jena, Germany

[4] BioteCon Gesell Biotechnol Entwicklung & Consulti, Berlin, Germany

[5] Robert Koch Inst, D-1000 Berlin, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 1999年 / 78卷 / 1-2期

关键词：

human cytomegalovirus; strains/variants; glycoprotein B; glycoprotein H; PCR-single strand polymorphism analysis; restriction fragment length polymorphism; liver transplantation;

D O I：

10.1016/S0166-0934(98)00173-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single strand conformation polymorphism analysis (SSCP) of PCR-amplified DNA and subsequent DNA sequencing of human cytomegalovirus (HCMV) glycoprotein B (gB) gene were applied to identify known HCMV strains and to detect new virus variants. 61 HCMV PCR positive patients were studied out of a cohort of 410 patients after liver transplantation (LTX). SSCP was able to distinguish between strains Davis, AD169, and Towne, and in addition could identify five new virus variants (Berlin B, C, E, F, and H). Their frequency, gB and gH types were determined. Simultaneous infections with two or three strains or variants, as well as a switch from one virus to another virus were observed during long-term follow-up. No correlation between the occurrence of certain Virus strains or gB types and defined clinical manifestations of HCMV infection after LTX was drawn. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 162

页数：10

共 25 条

[11] RAPID DETECTION OF HUMAN CYTOMEGALO-VIRUS DNA IN PERIPHERAL-BLOOD LEUKOCYTES OF VIREMIC TRANSPLANT RECIPIENTS BY THE POLYMERASE CHAIN-REACTION [J].