Enhancement of paclitaxel production by abscisic acid in cell suspension cultures of Taxus chinensis

被引：32

作者：

Luo, J ^{[1
]}

Liu, L ^{[1
]}

Wu, CD ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 16期

关键词：

abscisic acid; Paclitaxel; plant cell culture; Taxus chinensis;

D O I：

10.1023/A:1010597802741

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Addition of 5 mg abscisic acid l(-1) after 12 days' growth of Taxus chinensis suspension culture gave the greatest paclitaxel accumulation at 11 mg l(-1), which was almost 5 times that of the control culture. The highest paclitaxel production, 18 mg l(-1), was obtained using 5 mg abscisic acid l(-1) and 20 mg methyl jasmonate l(-1).

引用

页码：1345 / 1348

页数：4

共 19 条

[1] ABSCISIC-ACID ACCUMULATION IN LEAVES AND CULTURED-CELLS DURING HEAT ACCLIMATION IN GRAPES [J].

ABASS, M ;

RAJASHEKAR, CB .

HORTSCIENCE, 1993, 28 (01) :50-52

[2] Genetic analysis of ABA signal transduction pathways [J].

Bonetta, D ;

McCourt, P .

TRENDS IN PLANT SCIENCE, 1998, 3 (06) :231-235

[3]

Christen A.A., 1991, U.S. Patent, Patent No. [5,019,504, 5019504]

[4]

Ciddi V, 1995, BIOTECHNOL LETT, V17, P1343, DOI 10.1007/BF00189223

[5]

CLINTON CS, 1998, HICKSII YHEWS SUSTAI

[6] Production of Taxol® and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture [J].

Cusidó, RM ;

Palazón, J ;

Navia-Osorio, A ;

Mallol, A ;

Bonfill, M ;

Morales, C ;

Piñol, MT .

PLANT SCIENCE, 1999, 146 (02) :101-107

[7] IMPROVED TAXOL YIELD BY AROMATIC CARBOXYLIC-ACID AND AMINO-ACID FEEDING TO CELL-CULTURES OF TAXUS-CUSPIDATA [J].

FETTNETO, AG ;

MELANSON, SJ ;

NICHOLSON, SA ;

PENNINGTON, JJ ;

DICOSMO, F .

BIOTECHNOLOGY AND BIOENGINEERING, 1994, 44 (08) :967-971

[8] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].

GAMBORG, OL ;

MILLER, RA ;

OJIMA, K .

EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+

[9]

GIBSON DM, 1995, PACLITAXEL SCI APPL, P71

[10]

Ketchum REB, 1999, BIOTECHNOL BIOENG, V62, P97, DOI 10.1002/(SICI)1097-0290(19990105)62:1&lt

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