Differential effects of IL-2 and IL-15 on expression of IL-2 receptor α

被引：16

作者：

Alileche, A ^{[1
]}

Goldman, CK ^{[1
]}

Waldmann, TA ^{[1
]}

机构：

[1] NCI, Metab Branch, NIH, Bethesda, MD 20892 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 285卷 / 05期

关键词：

IL-2; IL-15; receptor; expression; transcription; translocation; cytokines; cytokines receptors; cellular activation; NK cells;

D O I：

10.1006/bbrc.2001.5332

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

IL-2 and IL-15 have overlapping functions since they share the IL-2R beta gamma receptor complex. However, each cytokine has a private a receptor namely IL-2R alpha for IL-2 and IL-15R alpha for IL-15. As a consequence the effects of the two cytokines may differ. We describe the differential effects of the two cytokines regarding the induction of cell surface expression of the IL-2R alpha subunit on YT-1 cells. Both cytokines induced transcription of the IL-2R alpha gene. Furthermore translation of IL-2R alpha leading to intracellular expression of the receptor was observed following either IL-2 or IL-15 addition. However, only IL-15 was associated with the induction of cell surface expression of IL-2R alpha. With IL-2 there appears to be an impediment to the translocation of IL-2R alpha to the cell membrane. Since surface expression of IL-2R alpha is a key element in the formation of the high affinity IL-2 receptor, translocation of IL-2R alpha to the membrane represents another level of control of the immune response in addition to regulation of IL-2R alpha transcription and translation.

引用

收藏

页码：1302 / 1308

页数：7

相关论文

共 44 条

[1]

ANDERSON DM, 1995, J BIOL CHEM, V270, P29862

[2]

[Anonymous], 1992, COMMUN THEOR

[3] A selective impairment of the IL-2 system in lymphocytes of patients with glioblastomas: Increased level of soluble IL-2R and reduced protein tyrosine phosphorylation [J].

Ashkenazi, E ;

Deutsch, M ;

Tirosh, R ;

Weinreb, A ;

Tsukerman, A ;

Brodie, C .

NEUROIMMUNOMODULATION, 1997, 4 (01) :49-56

[4] Interleukin (IL) 15/IL-T production by the adult T-cell leukemia cell line HuT-102 is associated with a human T-cell lymphotrophic virus type I R region/IL-15 fusion message that lacks many upstream AUGs that normally attenuate IL-15 mRNA translation [J].

Bamford, RN ;

Battiata, AP ;

Burton, JD ;

Sharma, H ;

Waldmann, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) :2897-2902

[5] THE INTERLEUKIN (IL)-2 RECEPTOR-BETA CHAIN IS SHARED BY IL-2 AND A CYTOKINE, PROVISIONALLY DESIGNATED IL-T, THAT STIMULATES T-CELL PROLIFERATION AND THE INDUCTION OF LYMPHOKINE-ACTIVATED KILLER-CELLS [J].

BAMFORD, RN ;

GRANT, AJ ;

BURTON, JD ;

PETERS, C ;

KURYS, G ;

GOLDMAN, CK ;

BRENNAN, J ;

ROESSLER, E ;

WALDMANN, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) :4940-4944

[6] IL-15: The role of translational regulation in their expression [J].

Bamford, RN ;

Battiata, AP ;

Waldmann, TA .

JOURNAL OF LEUKOCYTE BIOLOGY, 1996, 59 (04) :476-480

[7] Expression of the IL-2 receptor gamma subunit in resting human CD4 T lymphocytes: MRNA is constitutively transcribed and the protein stored as an intracellular component [J].

Bani, L ;

David, D ;

Moreau, JL ;

Cayota, A ;

Nakarai, T ;

Ritz, J ;

Theze, J .

INTERNATIONAL IMMUNOLOGY, 1997, 9 (04) :573-580

[8] A LYMPHOKINE, PROVISIONALLY DESIGNATED INTERLEUKIN-T AND PRODUCED BY A HUMAN ADULT T-CELL LEUKEMIA LINE, STIMULATES T-CELL PROLIFERATION AND THE INDUCTION OF LYMPHOKINE-ACTIVATED KILLER-CELLS [J].

BURTON, JD ;

BAMFORD, RN ;

PETERS, C ;

GRANT, AJ ;

KURYS, G ;

GOLDMAN, CK ;

BRENNAN, J ;

ROESSLER, E ;

WALDMANN, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) :4935-4939

[9] IRON-RESPONSIVE ELEMENTS - REGULATORY RNA SEQUENCES THAT CONTROL MESSENGER-RNA LEVELS AND TRANSLATION [J].

CASEY, JL ;

HENTZE, MW ;

KOELLER, DM ;

CAUGHMAN, SW ;

ROUAULT, TA ;

KLAUSNER, RD ;

HARFORD, JB .

SCIENCE, 1988, 240 (4854) :924-928

[10] Preassembly of interleukin 2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7, and IL-15: A fluorescence resonance energy transfer study [J].

Damjanovich, S ;

Bene, L ;

Matko, J ;

Alileche, A ;

Goldman, CK ;

Sharrow, S ;

Waldmann, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (24) :13134-13139

← 1 2 3 4 5 →