Fluorometric investigation on the interaction of oleanolic acid with bovine serum albumin

被引：50

作者：

Cheng, Zhengjun ^{[1
]}

Zhang, Yuntao ^{[1
]}

机构：

[1] China W Normal Univ, Inst Appl Chem, Nanchong 637002, Sichuan, Peoples R China

来源：

JOURNAL OF MOLECULAR STRUCTURE | 2008年 / 879卷 / 1-3期

关键词：

bovine serum albumin; oleanolic acid; fluorescence spectroscopy; circular dichroism spectroscopy; fourier transform infrared spectroscopy;

D O I：

10.1016/j.molstruc.2007.08.020

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The interactions between oleanolic acid and bovine serum albumin (BSA) have been studied by fluorescence, circular dichroism (CD), UV-vis absorption and Fourier transform infrared spectroscopy (FTIR) under physiological conditions. Spectroscopic analysis of the emission quenching at different temperatures has revealed that the quenching mechanism of bovine serum albumin by oleanolic acid is static quenching mechanism. The binding sites number n and binding constants K are obtained at various temperatures. The distance r between oleanolic acid and the protein is evaluated according to the theory of Forster energy transfer. The results by FTIR, CD and UV-vis absorption spectra experiment indicate that the secondary structures of protein have been perturbed in the presence of oleanolic acid. The thermodynamic parameters Delta H-0, Delta G(0), and Delta S-0 are calculated according to van't Hoff equation, which indicates that the hydrogen bonds and van der-waals are the intermolecular forces stabilizing the complex. Molecular modeling studies the interaction BSA with oleanolic acid. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：81 / 87

页数：7

共 34 条

[1] DSC STUDIES ON THE DENATURATION AND AGGREGATION OF SERUM ALBUMINS [J].

BARONE, G ;

GIANCOLA, C ;

VERDOLIVA, A .

THERMOCHIMICA ACTA, 1992, 199 :197-205

[2] Binding of anandamide to bovine serum albumin [J].

Bojesen, IN ;

Hansen, HS .

JOURNAL OF LIPID RESEARCH, 2003, 44 (09) :1790-1794

[3] 3-DIMENSIONAL STRUCTURE OF HUMAN-SERUM ALBUMIN [J].

CARTER, DC ;

HE, XM ;

MUNSON, SH ;

TWIGG, PD ;

GERNERT, KM ;

BROOM, MB ;

MILLER, TY .

SCIENCE, 1989, 244 (4909) :1195-1198

[4]

CISTOLA DP, 1987, J BIOL CHEM, V262, P10980

[5] Fluorescent investigation of the interactions between N-(p-chlorophenyl)-N'-(1-naphthyl) thiourea and serum albumin:: Synchronous fluorescence determination of serum albumin [J].

Cui, Feng-Ling ;

Wang, Jun-Li ;

Cui, Yan-Rul ;

Li, J'an-Ping .

ANALYTICA CHIMICA ACTA, 2006, 571 (02) :175-183

[6] Interactions between 1-benzoyl-4-p-chlorophenyl thiosemicarbazide and serum albumin:: investigation by fluorescence spectroscopy [J].

Cui, FL ;

Fan, J ;

Li, JP ;

Hu, ZD .

BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (01) :151-157

[7]

Forster T., 1996, MODERN QUANTUM CHEM, V3, P93

[8] Interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants: spectroscopy and modelling [J].

Gelamo, EL ;

Silva, CHTP ;

Imasato, H ;

Tabak, M .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1594 (01) :84-99

[9] Small-angle X-ray scattering and electron paramagnetic resonance study of the interaction of bovine serum albumin with ionic surfactants [J].

Gelamo, EL ;

Itri, R ;

Alonso, A ;

da Silva, JV ;

Tabak, M .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2004, 277 (02) :471-482

[10] LOCATIONS OF THE 3 PRIMARY BINDING-SITES FOR LONG-CHAIN FATTY-ACIDS ON BOVINE SERUM-ALBUMIN [J].

HAMILTON, JA ;

ERA, S ;

BHAMIDIPATI, SP ;

REED, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2051-2054

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