Immunological identification of the mammalian H3 histamine receptor in the mouse brain

Affinity-purified antibodies raised against the peptide sequence H-3 (349-358) receptor specifically recognized Mo protein species with M-r 62 000 and 93 000 in adult mouse forebrain membranes. Both immunoreactive species were suppressed greatly by preincubation of the antibody with the respective peptide. Immunohistochemical analysis using affinity-purified anti-H-3 (349-358) antibodies yielded a high degree of coincidence with ligand-autoradiographical information, with high levels detected in the CA3 and dentate gyrus of the hippocampus, laminae V of the cerebral cortex, the olfactory tubercle. Purkinje cell layer of the cerebellum, substantia nigra, globus pallidus, thalamus and striatum. This study suggests further biochemical evidence for multiple H-3 receptor subtypes and the widespread distribution of-the H-3 receptor in the mammalian brain. NeuroReport 12:259-262 (C) 2001 Lippincott Williams & Wilkins.