Extracellular glycosyl hydrolase activity of the Clostridium strains producing acetone, butanol, and ethanol

被引:13
作者
Berezina, O. V. [1 ]
Sineoky, S. P. [2 ]
Velikodvorskaya, G. A. [1 ]
Schwarz, W. [3 ]
Zverlov, V. V. [1 ]
机构
[1] Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia
[2] Moscow Genet & Select Ind Microorganisms Inst, Moscow 117545, Russia
[3] Tech Univ Munich, Inst Microbiol, D-85350 Freising Weihenstephan, Germany
关键词
D O I
10.1134/S0003683808010079
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Production of acetone, butanol, ethanol, acetic acid, and butyric acid by three strains of anaerobic bacteria, which we identified as Clostridium acetobutylicum, was studied. The yield of acetone and alcohols in 6% wheat flour medium amounted to 12.7-15 g/l with butanol constituting 51.0-55.6%. Activities of these strains towards xylan, beta-glucan, carboxymethylcellulose, and crystalline and amorphous celluloses were studied. C. acetobutylicum 6, C. acetobutylicum 7, and C. acetobutylicum VKPM B-4786 produced larger amounts of acetone and alcohols and displayed higher cellulase and hemicellulase activities than the type strain C. acetobutylicum ATCC 824 in lab-scale butch cultures. It was demonstrated that starch in the medium could be partially substituted with plant biomass.
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页码:42 / 47
页数:6
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