Natriuretic peptides and immunoreactants modify osmoticum-dependent volume changes in Solanum tuberosum L. mesophyll cell protoplasts

被引:33
作者
Maryani, MM
Bradley, G
Cahill, DM
Gehring, CA
机构
[1] Univ Western Cape, Dept Biochem, ZA-7535 Bellville, South Africa
[2] Deakin Univ, Sch Biol & Chem Sci, Geelong, Vic 3217, Australia
基金
澳大利亚研究理事会;
关键词
peptides; protoplasts; hormones;
D O I
10.1016/S0168-9452(01)00423-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In plants, as in vertebrates, natriuretic peptide (NP) hormones can influence water and solute homeostasis. Here we demonstrate that a synthetic peptide identical to the C-terminus (amino acids 99-126) of the rat atrial natriuretic peptide (rANP) modulates osmotically induced swelling of mesophyll cell protoplasts (MCPs) in a concentration and time-dependent manner. Osmotically-induced volume changes in MCPs are enhanced by plant extracts with NP immunoreactivity and this effect is concentration-dependent. In contrast, pre-treatment of the plant extracts with rabbit anti-human ANP (99-126) antiserum Suppresses enhanced osmoticum-induced swelling. Isolated plant peptides (irPNP) that have been immunoaffinity purified with rabbit anti-human ANP (99-126) antiserum also enhance osmotically-induced swelling. While rANP and irPNP cause increases in cGMP levels in MCPs, elevated cGMP levels do not cause increases in osmoticum-dependent swelling but exert an inhibitory effect. These findings are consistent with a NP-dependent, cGMP-independent effect on plant cell volume regulation and a role in homeostasis for peptides that are recognized by antibodies directed against the C-terminus of vertebrate ANPs. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:443 / 452
页数:10
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