Cytomegalovirus glycoprotein B sequence variation among Japanese bone marrow transplant recipients

被引:24
作者
Wada, K
Mizuno, S
Kato, K
Kamiya, T
Ozawa, K
机构
[1] Daido Hosp, Minami Ku, Nagoya, Aichi 457, Japan
[2] Aichi Red Cross, Ctr Blood, Seto, Japan
[3] Nagoya First Hosp, Japan Red Cross, Childrens Med Ctr, Div Hematol Oncol, Nagoya, Aichi, Japan
关键词
cytomegalovirus; human; glycoprotein B variation; bone marrow transplantation;
D O I
10.1159/000150549
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Glycoprotein B (gB) of human cytomegalovirus (CMV) is the major target protein of neutralizing antibodies, and four variant types are known. A previously reported polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method was modified by eliminating the cell culture step and amplifying a CMV fragment (corresponding to a.a. 441-450 of gB) by nested PCR. DNA was extracted from the sera of 27 pediatric bone marrow transplant recipients and subjected to nested PCR. Of the samples, 20 yielded PCR products, and the gB type was determined by RFLP. Of the 20 patients, 4 (20%) had gB type 1 (Towne type), 15 patients (75%) had gB type 2 (AD 169 type), and 1 patient (5 %) had gB type 3. Previous studies showed that gB type was most common among Caucasians [Chou and Dennison: J Infect Dis 1991;163:1229-1234; Fries et al: J Infect Dis 1994;169:769-774]. Thus, gB genotypes seem to be distributed differently in Caucasians and Japanese. Further, nucleotide sequence analysis of the amplified region revealed that all the type 2 viruses had the same amino acid sequences. The type 3 sample had a novel amino acid substitution at position 498. Of the type 1 samples, 3 had amino acid substitutions in various positions: 1 sample at position 493, 1 sample at position 447 and 1 sample at position 452, 493 and 498.
引用
收藏
页码:215 / 219
页数:5
相关论文
共 20 条
[1]  
CHEE MS, 1990, CURR TOP MICROBIOL, V154, P125
[2]   ACQUISITION OF DONOR STRAINS OF CYTOMEGALOVIRUS BY RENAL-TRANSPLANT RECIPIENTS [J].
CHOU, SW .
NEW ENGLAND JOURNAL OF MEDICINE, 1986, 314 (22) :1418-1423
[3]   ANALYSIS OF INTERSTRAIN VARIATION IN CYTOMEGALOVIRUS GLYCOPROTEIN-B SEQUENCES ENCODING NEUTRALIZATION-RELATED EPITOPES [J].
CHOU, SW ;
DENNISON, KM .
JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (06) :1229-1234
[4]   MOLECULAR EPIDEMIOLOGY OF ENVELOPE GLYCOPROTEIN-H OF HUMAN CYTOMEGALOVIRUS [J].
CHOU, SW .
JOURNAL OF INFECTIOUS DISEASES, 1992, 166 (03) :604-607
[5]   NEUTRALIZING ANTIBODY-RESPONSES TO REINFECTING STRAINS OF CYTOMEGALO-VIRUS IN TRANSPLANT RECIPIENTS [J].
CHOU, SW .
JOURNAL OF INFECTIOUS DISEASES, 1989, 160 (01) :16-21
[6]   COMPARATIVE-ANALYSIS OF SEQUENCE VARIATION IN GP116 AND GP55 COMPONENTS OF GLYCOPROTEIN-B OF HUMAN CYTOMEGALOVIRUS [J].
CHOU, SW .
VIROLOGY, 1992, 188 (01) :388-390
[8]   IDENTIFICATION OF THE HUMAN CYTOMEGALOVIRUS GLYCOPROTEIN-B GENE AND INDUCTION OF NEUTRALIZING ANTIBODIES VIA ITS EXPRESSION IN RECOMBINANT VACCINIA VIRUS [J].
CRANAGE, MP ;
KOUZARIDES, T ;
BANKIER, AT ;
SATCHWELL, S ;
WESTON, K ;
TOMLINSON, P ;
BARRELL, B ;
HART, H ;
BELL, SE ;
MINSON, AC ;
SMITH, GL .
EMBO JOURNAL, 1986, 5 (11) :3057-3063
[9]  
FORMAN SJ, 1994, BLOOD, V83, P2392
[10]   FREQUENCY-DISTRIBUTION OF CYTOMEGALOVIRUS ENVELOPE GLYCOPROTEIN GENOTYPES IN BONE-MARROW TRANSPLANT RECIPIENTS [J].
FRIES, BC ;
CHOU, SW ;
BOECKH, M ;
TOROKSTORB, B .
JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (04) :769-774