Long-term postmortem survival of mitochondrial genomes in mouse synaptosomes and their rescue in a mitochondrial DNA-less mouse cell line

被引：9

作者：

Ito, S

Inoue, K

Yanagisawa, N

Kaneko, M

Hayashi, JI ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Biol Sci, Ibaraki, Osaka 3058572, Japan

[2] Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Ibaraki, Osaka 3058572, Japan

[3] Natl Canc Ctr, Res Inst, Div Biophys, Chuo Ku, Tokyo 1040045, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 247卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.8800

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondrial DNA (mtDNA) transfer was carried out from postmortem mouse tissues to mouse mtDNA-less (rho(0)) cells to determine how long it takes for autolysis of mtDNA after death and whether mtDNA in postmortem tissues can recover its function in rho(0) cells. The results showed that mtDNA was stable in postmitotic tissues stored at 4 degrees C. Moreover, mtDNA in postmortem brain tissues stored for up to 1 month still retained functional properties, causing complete recovery of mitochondrial respiratory function, when it was transferred to rho(0) cells. These observations suggest that mtDNA in brain tissue can survive for 1 month after death and can start replication and gene expression in rho(0) cells without showing any functional defects. This procedure might be applied to human autopsy brain tissues for examination of the influence of accumulated somatic mutations in mtDNA from aged subjects. (C) 1998 Academic Press.

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收藏

页码：432 / 435

页数：4

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