Constitutively enhanced nbl expression is associated with the induction of internucleosomal DNA cleavage by actinomycin D

被引：27

作者：

Naora, H ^{[1
]}

Nishida, T ^{[1
]}

Shindo, Y ^{[1
]}

Adachi, M ^{[1
]}

Naora, H ^{[1
]}

机构：

[1] OTSUKA PHARMACEUT CO LTD,CELLULAR TECHNOL INST,TOKUSHIMA,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 224卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.1017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have found nbl expression to transiently rise and fall during gluco corticoid-induced thymic apoptosis. This induction of apoptosis is blocked by the transcriptional inhibitor actinomycin D. However, actinomycin D can trigger apoptosis in other cell types, e.g., HL-60 cells. This study found that internucleosomal DNA cleavage typical of apoptosis is induced by actinomycin D in cell lines such as HL-60 which constitutively express high levels of nbl above a certain ''threshold'' In contrast, ''DNA ladder'' formation was not induced by actinomycin D in cell lines with low constitutive nbl expression, Enhanced nbl expression therefore appears to be associated with apoptosis which is either blocked or induced by actinomycin D. (C) 1996 Academic Press, Inc.

引用

页码：258 / 264

页数：7

共 18 条

[1] THE HUMAN UBIQUITIN GENE FAMILY - STRUCTURE OF A GENE AND PSEUDOGENES FROM THE UB-B SUBFAMILY [J].

BAKER, RT ;

BOARD, PG .

NUCLEIC ACIDS RESEARCH, 1987, 15 (02) :443-463

[2]

DESAJRDINS LM, 1995, EXP CELL RES, V216, P380

[3] ENDOGENOUS ENDONUCLEASE-INDUCED DNA FRAGMENTATION - AN EARLY EVENT IN CELL-MEDIATED CYTOLYSIS [J].

DUKE, RC ;

CHERVENAK, R ;

COHEN, JJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (20) :6361-6365

[4] FTE-1, A V-FOS TRANSFORMATION EFFECTOR GENE, ENCODES THE MAMMALIAN HOMOLOG OF A YEAST GENE INVOLVED IN PROTEIN IMPORT INTO MITOCHONDRIA [J].

KHO, CJ ;

ZARBL, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2200-2204

[5] IMPLICATION OF MAMMALIAN RIBOSOMAL-PROTEIN S3 IN THE PROCESSING OF DNA-DAMAGE [J].

KIM, J ;

CHUBATSU, LS ;

ADMON, A ;

STAHL, J ;

FELLOUS, R ;

LINN, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (23) :13620-13629

[6] METHYLATION MAP OF XENOPUS-LAEVIS RIBOSOMAL-RNA [J].

MADEN, BEH .

NATURE, 1980, 288 (5788) :293-296

[7]

Martin Seamus J., 1993, Trends in Cell Biology, V3, P141, DOI 10.1016/0962-8924(93)90128-N

[8]

MARTIN SJ, 1990, J IMMUNOL, V145, P1859

[9] VARIABLE SUSCEPTIBILITY TO APOPTOSIS INDUCED BY CALCIUM IONOPHORE IN HYBRIDOMAS BETWEEN HL-60 PROMYELOCYTIC AND CEM T-LYMPHOBLASTIC LEUKEMIA-CELL LINES - RELATIONSHIP TO CONSTITUTIVE MG2+-DEPENDENT ENDONUCLEASE [J].

MATSUBARA, K ;

KUBOTA, M ;

KUWAKADO, K ;

HIROTA, H ;

WAKAZONO, Y ;

OKUDA, A ;

BESSHO, R ;

LIN, YW ;

ADACHI, S ;

AKIYAMA, Y .

EXPERIMENTAL CELL RESEARCH, 1994, 213 (02) :412-417

[10] HUMAN RIBOSOMAL PROTEIN-S3A - CLONING OF THE CDNA AND PRIMARY STRUCTURE OF THE PROTEIN [J].

METSPALU, A ;

REBANE, A ;

HOTH, S ;

POOGA, M ;

STAHL, J ;

KRUPPA, J .

GENE, 1992, 119 (02) :313-316

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