Heterogeneous nuclear ribonuclear protein K interacts with Sindbis virus nonstructural proteins and viral subgenomic mRNA

被引：74

作者：

Burnham, Andrew J. ^{[1
]}

Gong, Lei ^{[1
]}

Hardy, Richard W. ^{[1
]}

机构：

[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

来源：

VIROLOGY | 2007年 / 367卷 / 01期

关键词：

hnRNP K; Sindbis virus; nonstructural proteins; subgenomic mRNA;

D O I：

10.1016/j.virol.2007.05.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Alphaviruses are a group of arthropod-borne human and animal pathogens that can cause epidemics of significant public health and economic consequence. Alphavirus RNA synthesis requires four virally encoded nonstructural proteins and probably a number of cellular proteins. Using comparative two-dimensional electrophoresis we were able to identify proteins enriched in cytoplasmic membrane fractions containing viral RNA synthetic complexes following infection with Sindbis virus. Our studies demonstrated the following: (i) the host protein hnRNP K is enriched in cytoplasmic membrane fractions following Sindbis virus infection, (ii) viral nonstructural proteins co-immunoprecipitate with hnRNP K, (iii) nsP2 and hnRNP K co-localize in the cytoplasm of Sindbis virus infected cells, (iv) Sindbis virus subgenomic mRNA, but not genomic RNA co-immunoprecipitates with hnRNP K, (v) viral RNA does not appear to be required for the interaction of hnRNP K with the nonstructural proteins. Potential functions of hnRNP K during virus replication are discussed. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：212 / 221

页数：10

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