Fourier transform infrared spectroscopic analysis of protein secondary structures

被引：3018

作者：

Kong, Jilie ^{[1
]}

Yu, Shaoning ^{[1
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2007年 / 39卷 / 08期

关键词：

FTIR; protein structure; protein dynamic;

D O I：

10.1111/j.1745-7270.2007.00320.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its applications to protein structural studies. The experimental skills, data analysis, and correlations between the FTIR spectroscopic bands and protein secondary structure components are discussed. The applications of FTIR to the secondary structure analysis, conformational changes, structural dynamics and stability studies of proteins are also discussed.

引用

页码：549 / 559

页数：11

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