Novel fluorogenic substrates for imaging 6-lactamase gene expression

被引：170

作者：

Gao, WZ

Xing, BG

Tsien, RY

Rao, JH ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Crump Inst Mol Imaging, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[2] Univ Calif San Diego, Howard Hughes Med Inst, Dept Pharmacol, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Howard Hughes Med Inst, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 37期

关键词：

D O I：

10.1021/ja036126o

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after β-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of β-lactamase in cell lysates can be readily detected, and β-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of β-lactamase as a biosensor. Copyright © 2003 American Chemical Society.

引用

页码：11146 / 11147

页数：2

共 15 条

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