Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)

被引：321

作者：

Sander, Jeffry D. ^{[1
,2
,3
]}

Dahlborg, Elizabeth J. ^{[1
,2
]}

Goodwin, Mathew J. ^{[1
,2
]}

Cade, Lindsay ^{[4
]}

Zhang, Feng ^{[5
,6
]}

Cifuentes, Daniel ^{[7
]}

Curtin, Shaun J. ^{[8
]}

Blackburn, Jessica S. ^{[1
,2
,3
]}

Thibodeau-Beganny, Stacey ^{[1
,2
]}

Qi, Yiping ^{[5
,6
]}

Pierick, Christopher J. ^{[5
,6
]}

Hoffman, Ellen ^{[7
]}

Maeder, Morgan L. ^{[1
,2
,9
]}

Khayter, Cyd ^{[1
,2
]}

Reyon, Deepak ^{[10
,11
]}

Dobbs, Drena ^{[10
,11
]}

Langenau, David M. ^{[1
,3
,9
]}

Stupar, Robert M. ^{[8
]}

Giraldez, Antonio J. ^{[7
]}

Voytas, Daniel F. ^{[5
,6
]}

Peterson, Randall T. ^{[4
,12
,13
]}

Yeh, Jing-Ruey J. ^{[4
,12
]}

Joung, J. Keith ^{[1
,2
,3
,9
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Canc Res, Charlestown, MA USA

[2] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Charlestown, MA USA

[3] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[4] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Charlestown, MA USA

[5] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA

[6] Univ Minnesota, Ctr Genome Engn, Minneapolis, MN USA

[7] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06510 USA

[8] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA

[9] Harvard Univ, Sch Med, Biol & Biomed Sci Program, Boston, MA USA

[10] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA

[11] Iowa State Univ, Bioinformat & Computat Biol Program, Ames, IA USA

[12] Harvard Univ, Sch Med, Dept Med, Boston, MA USA

[13] Broad Inst, Cambridge, MA USA

来源：

NATURE METHODS | 2011年 / 8卷 / 01期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

TARGETED MUTAGENESIS;

D O I：

10.1038/NMETH.1542

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe context-dependent assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA-generated ZFNs, we rapidly altered 20 genes in Danio rerio, Arabidopsis thaliana and Glycine max. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multigene pathways or genome-wide alterations.

引用

页码：67 / U94

页数：4

共 20 条

[1] Progress and prospects: Zinc-finger nucleases as gene therapy agents [J].

Carroll, D. .

GENE THERAPY, 2008, 15 (22) :1463-1468

[2] Zinc-finger nucleases: The next generation emerges [J].

Cathomen, Toni ;

Joung, J. Keith .

MOLECULAR THERAPY, 2008, 16 (07) :1200-1207

[3] Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases [J].

Doyon, Yannick ;

McCammon, Jasmine M. ;

Miller, Jeffrey C. ;

Faraji, Farhoud ;

Ngo, Catherine ;

Katibah, George E. ;

Amora, Rainier ;

Hocking, Toby D. ;

Zhang, Lei ;

Rebar, Edward J. ;

Gregory, Philip D. ;

Urnov, Fyodor D. ;

Amacher, Sharon L. .

NATURE BIOTECHNOLOGY, 2008, 26 (06) :702-708

[4] Targeted mutagenesis in zebrafish using customized zinc-finger nucleases [J].

Foley, Jonathan E. ;

Maeder, Morgan L. ;

Pearlberg, Joseph ;

Joung, J. Keith ;

Peterson, Randall T. ;

Yeh, Jing-Ruey J. .

NATURE PROTOCOLS, 2009, 4 (12) :1855-1868

[5] Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN) [J].

Foley, Jonathan E. ;

yeh, Jing-Ruey J. ;

Maeder, Morgan L. ;

Reyon, Deepak ;

Sander, Jeffry D. ;

Peterson, Randall T. ;

Joung, J. Keith .

PLOS ONE, 2009, 4 (02)

[6] Evaluation of constitutive viral promoters in transgenic soybean roots and nodules [J].

Govindarajulu, Manjula ;

Elmore, James M. ;

Fester, Thomas ;

Taylor, Christopher G. .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2008, 21 (08) :1027-1035

[7] Comprehensive DNA recognition through concerted interactions from adjacent zinc fingers [J].

Isalan, M ;

Klug, A ;

Choo, Y .

BIOCHEMISTRY, 1998, 37 (35) :12026-12033

[8] Synergy between adjacent zinc fingers in sequence-specific DNA recognition [J].

Isalan, M ;

Choo, Y ;

Klug, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (11) :5617-5621

[9] Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly [J].

Kim, Hye Joo ;

Lee, Hyung Joo ;

Kim, Hyojin ;

Cho, Seung Woo ;

Kim, Jin-Soo .

GENOME RESEARCH, 2009, 19 (07) :1279-1288

[10] Genome editing with modularly assembled zinc-finger nucleases [J].

Kim, Jin-Soo ;

Lee, Hyung Joo ;

Carroll, Dana .

NATURE METHODS, 2010, 7 (02) :91-91

← 1 2 →