Quantum dots as a visual aid for screening bead-bound combinatorial libraries

被引：52

作者：

Olivos, HI

Bachhawat-Sikder, K

Kodadek, T

机构：

[1] Univ Texas, SW Med Ctr, Ctr Biomed Invent, Dallas, TX 75390 USA

[2] Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75390 USA

[3] Univ Texas, SW Med Ctr, Dept Mol Biol, Dallas, TX 75390 USA

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 11期

关键词：

D O I：

10.1002/cbic.200300712

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seeing red: A new methodology for the on-bead screening of proteins has been developed with the aid of red-emitting quantum dots. Use of this nanomaterial allowed a dual-color fluorescence assay to be developed that overcomes the limitation of the autofluorescence of TentaGel beads. The figure shows a positive control (red fluorescence) and a negative control (background green fluorescence).

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页码：1242 / 1245

页数：4

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