Unzipping kinetics of double-stranded DNA in a nanopore

被引：250

作者：

Sauer-Budge, AF ^{[1
]}

Nyamwanda, JA

Lubensky, DK

Branton, D

机构：

[1] Harvard Univ, Bioengn Program, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[3] Lucent Technol, Bell Labs, Murray Hill, NJ 07974 USA

来源：

PHYSICAL REVIEW LETTERS | 2003年 / 90卷 / 23期

关键词：

D O I：

10.1103/PhysRevLett.90.238101

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We studied the unzipping of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. Polymerase chain reaction analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.

引用

页数：4

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