Capture of a single molecule in a nanocavity

被引：128

作者：

Gu, LQ

Cheley, S

Bayley, H ^{[1
]}

机构：

[1] Texas A&M Univ, Syst Hlth Sci Ctr, Dept Med Biochem & Genet, College Stn, TX 77843 USA

[2] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA

来源：

SCIENCE | 2001年 / 291卷 / 5504期

关键词：

D O I：

10.1126/science.291.5504.636

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a heptameric protein pore that has been engineered to accommodate two different cyclodextrin adapters simultaneously within the Lumen of a transmembrane beta barrel. The volume between the adapters is a cavity of similar to 4400 cubic angstroms. Analysis of single-channel recordings reveals that individual charged organic molecules can be pulled into the cavity by an electrical potential. Once trapped, an organic molecule shuttles back and forth between the adapters for hundreds of milliseconds. Such self-assembling nanostructures are of interest for the fabrication of multianalyte sensors and could provide a means to control chemical reactions.

引用

页码：636 / 640

页数：5

共 19 条

[1]

Bayley H, 2000, ADV MATER, V12, P139, DOI 10.1002/(SICI)1521-4095(200001)12:2<139::AID-ADMA139>3.3.CO

[2]

2-H

[3] Simultaneous stochastic sensing of divalent metal ions [J].