Isolation and characterization of phenol-catabolizing bacteria from a coking plant

被引:40
作者
El-Sayed, WS
Ibrahim, MK
Abu-Shady, M
El-Beih, F
Ohmura, N
Saiki, H
Ando, A
机构
[1] Cent Res Inst Elect Power Ind, Dept Biosci, Abiko, Chiba 2701194, Japan
[2] Ain Shams Univ, Fac Sci, Dept Microbiol, Cairo, Egypt
[3] Chiba Univ, Dept Sci & Technol, Chiba 2718510, Japan
关键词
phenol; degradation; tolerance; meta-pathway;
D O I
10.1271/bbb.67.2026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
New phenol degrading bacteria with high biodegradation activity and high tolerance were isolated as Burkholderia cepacia PW3 and Pseudomonas aeruginosa AT2. Both isolates could grow aerobically on phenol as a sole carbon source even at 3 g/l. The whole-cell kinetic properties for phenol degradation by strains PW3 and AT2 showed a V-max of 0.321 and 0.253 mg/l/min/(mg protein), respectively. The metabolic pathways for phenol biodegradation in both strains were assigned to the meta-cleavage activity of catechol 2,3-dioxygenase.
引用
收藏
页码:2026 / 2029
页数:4
相关论文
共 14 条
[1]  
[Anonymous], 1993, Bergey's Manual of Determinative Bacteriology
[2]  
[Anonymous], 1983, COLD SPRING HARBOR L
[3]   Kinetics of phenol degradation using Pseudomonas putida MTCC 1194 [J].
Bandyopadhyay, K ;
Das, D ;
Maiti, BR .
BIOPROCESS ENGINEERING, 1998, 18 (05) :373-377
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   A new bioreactor with a semi-fixed packing: Investigation of degradation of phenol [J].
Daraktchiev, R ;
Kolev, N ;
Beschkov, V ;
Aleksandrova, T .
BIOPROCESS ENGINEERING, 1996, 16 (01) :5-7
[6]   Degradation of mono-chlorophenols by a mixed microbial community via a meta-cleavage pathway [J].
Farrell, A ;
Quilty, B .
BIODEGRADATION, 1999, 10 (05) :353-362
[7]   PHENOL AND TRICHLOROETHYLENE DEGRADATION BY PSEUDOMONAS-CEPACIA G4 - KINETICS AND INTERACTIONS BETWEEN SUBSTRATES [J].
FOLSOM, BR ;
CHAPMAN, PJ ;
PRITCHARD, PH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (05) :1279-1285
[8]   Diversity in kinetics of trichloroethylene-degrading activities exhibited by phenol-degrading bacteria [J].
Futamata, H ;
Harayama, S ;
Watanabe, K .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 55 (02) :248-253
[9]   Accumulation of plant galactolipid affects cell morphology of Escherichia coli [J].
Gad, M ;
Awai, K ;
Shimojima, M ;
Yamaryo, Y ;
Shimada, H ;
Masuda, T ;
Takamiya, K ;
Ikai, A ;
Ohta, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 286 (01) :114-118
[10]  
Greenberg A. E., 1992, STANDARD METHODS EXA