The promoter of human p22/PACAP response gene 1 (PRG1) contains functional binding sites for the p53 tumor suppressor and for NFκB

We describe functional binding sites for the tumor suppressor p53 and for NF kappa B residing in the promoter of the novel human early response gene p22/PRG1 (IEX-1/DLF-2). Gel shift and supershift assays demonstrate binding of p53 and NF kappa B to their corresponding sites in vitro. CAT-reporter gene assays show transactivation of the human p22/PRG1 promoter by p53 in Hep3B cells stably transfected with a temperature-sensitive mutant p53, but not in p53-deficient Hep3B cells. TNF alpha induced NF kappa B dependent transactivation was shown in HepG2 cells or in 818-4 pancreatic cancer cells. These data imply that human p22/PRG1 is a target gene for p53 and NF kappa B invoiced in growth regulation and stress response. (C) 1998 Federation of European Biochemical Societies.