Synergy between Lactobacillus paracasei and its bacterial products to counteract stress-induced gut permeability and sensitivity increase in rats

被引:131
作者
Eutamene, Helene
Lamine, Florence
Chabo, Chantal
Theodorou, Vassilia
Rochat, Florence
Bergonzelli, Gabriela E.
Corthesy-Theulaz, Irene
Fioramonti, Jean
Bueno, Lionel [1 ]
机构
[1] INRA, EI Purpan, UMR 1054, Neurogastroenterol & Nutr Unit, F-31931 Toulouse, France
[2] Nestle Res Ctr, CH-1000 Lausanne, Switzerland
关键词
D O I
10.1093/jn/137.8.1901
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Stressful events result in the alteration of gut permeability and sensitivity. Lactobacillus paracasei NCC2461 (Lpa) therapy prevents antibiotic-induced visceral hyperalgesia in mice. This study aimed at evaluating the influence of 3 probiotic strains: Bifidobacterium lactis NCC362, Lactobacillus johnsonii NCC533, and Lpa on stress-mediated alterations of colorectal hyperalgesia, on gut paracellular permeability and whether bacteria and/or bacterial products present in the spent culture medium (SCM) were involved in the antinociceptive properties of the effective strain. Rat pups were separated from their mothers 3 h/d during postnatal d 2-14. At wk 13, gut paracellular permeability was determined as a percentage of urinary excreted Cr-51-EDTA and visceral sensitivity to colorectal distension (CRD), assessed by abdominal muscle electromyography. Visceral sensitivity was also analyzed in adults rats subjected to partial restraint stress (PRS, 2 h restriction of body movements). Rats received either the probiotics resuspended in SCM or fresh growth medium as control for 2 wk. Maternal deprivation significantly increased colonic sensitivity in response to CRD and enhanced gut paracellular permeability compared with control rats. Only Lpa treatment significantly improved stress-induced visceral pain and restored normal gut permeability. Similarly, among the 3 probiotics tested, only Lpa prevented PRS-mediated visceral hyperalgesia. Both bacteria and bacterial products present in Lpa SCM were required for the antinociceptive properties against PRS. This study illustrates strain-specific effects arid suggests a synergistic interplay between L. paracasei bacteria and bacterial products generated during fermentation arid growth that confers the ability to suppress PRS-induced hypersensitivity in rats.
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页码:1901 / 1907
页数:7
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