Development of agents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining

被引：14

作者：

Ito, S ^{[1
]}

Muguruma, N

Hayashi, S

Taoka, S

Bando, T

Inayama, K

Sogabe, M

Okahisa, T

Okamura, S

Shibata, H

Irimura, T

Takesako, K

Shibamura, S

机构：

[1] Univ Tokushima, Sch Med, Dept Internal Med 2, Tokushima 7708503, Japan

[2] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[3] Dojindo Labs, Kumamoto 8612202, Japan

[4] Daiichi Pure Chem Co Ltd, Chuo Ku, Tokyo 1030029, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1998年 / 6卷 / 05期

关键词：

reinforcement agents; fluorescence; infrared rays; distearoylphosphatidic acid sodium salt (DSPA); octylglucoside (OG);

D O I：

10.1016/S0968-0896(98)00023-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Fluorescence intensity of indocyanine green (ICG) derivative (ICG-sulfo-OSu) was too low for its use to detect microlesions. Therefore, we examined the effects of reinforcement agents on ICG-sulfo-OSu labeled antibodies. Solutions of distearoylphosphatic acid sodium salt (DSPA) and octylglucoside (OG) in physiological phosphate buffered saline (PBS) were found to increase the intensity of fluorescence of ICG-sulfo-OSu labeled antibodies, with shift in the fluorescence peak wavelength from 804 to 821 nm. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：613 / 618

页数：6

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