Effects of Carolina rinse solution, dimethyl sulfoxide, and the 21-aminosteroid, U-74389G, on microvascular permeability and morphology of the equine jejunum after low-flow ischemia and reperfusion

被引:20
作者
Dabareiner, RM [1 ]
White, NA
Snyder, JR
Feldman, BF
Donaldson, LL
机构
[1] Virginia Polytech Inst & State Univ, Marion DuPont Scott Equine Med Ctr, Leesburg, VA 20177 USA
[2] Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Leesburg, VA 20177 USA
[3] Univ Calif Davis, Dept Surg, Davis, CA 95616 USA
[4] Univ Calif Davis, Dept Radiol Sci, Sch Vet Med, Davis, CA 95616 USA
关键词
D O I
10.2460/ajvr.2005.66.525
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 [兽医学];
摘要
Objective-To evaluate effects of Carolina rinse solution, dimethyl sulfoxide (DMSO), and 21-amino-steroid, U-74389G, on microvascular permeability and morphology of the equine jejunum after low-flow ischemia and reperfusion. Animals-20 healthy adult horses. Procedure-Under anesthesia, full-thickness biopsy specimens of a distal portion of the jejunum were obtained for baseline measurements. In addition to a control segment, 2 jejunal segments were identified as sham-operated or experimental segments. Experimental segments underwent 60 minutes of low-flow ischemia and 3.5 hours of reperfusion. Treatments were as follows: U-74389G (3 mg/kg, IV; 6 horses), DMSO (20 mg/kg, IV; 6) diluted in 1 L of saline (0.9% NaCl) solution, local perfusion (via jejunal artery) of Carolina rinse solution (0.5 mL/kg; 4), and local perfusion of lactated Ringer's solution (0.5 mL/kg; 4). Results-Jejunal microvascular permeability was significantly lower after treatment with Carolina rinse solution or DMSO, compared with U-74389G or lactated Ringer's solution treatments. After DMSO treatment, serosal- and submucosal-layer edema was significantly increased in experimental segments, compared with control or sham-operated segments; however, edema increases were significantly less than for lactated Ringer's solution or U-74389G treatments. Significant decreases in intestinal wet weight-to-dry weight ratio were found following Carolina rinse solution or DMSO treatments, compared with lactated Ringer's solution or U-74389G treatments. Edema formation and leukocyte infiltration in jejunal segments of horses treated with lactated Ringer's solution or U-74389G were increased, compared with Carolina rinse solution or DMSO treatments. Conclusions and Clinical Relevance-Carolina rinse solution and DMSO may be protective against ischemia-reperfusion injury in the equine jejunum.
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收藏
页码:525 / 536
页数:12
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