An improved technique for the assay of red blood cell superoxide dismutase (SOD) activity

被引：16

作者：

Ilouno, LE ^{[1
]}

Shu, EN ^{[1
]}

Igbokwe, GE ^{[1
]}

机构：

[1] NNAMDI AZIKWE UNIV,DEPT APPL BIOCHEM,AWK,NIGERIA

来源：

CLINICA CHIMICA ACTA | 1996年 / 247卷 / 1-2期

关键词：

superoxide dismutase; pre-eclamptic toxaemia; pregnancy; nitroblue tetrazolium; red blood cells;

D O I：

10.1016/0009-8981(95)06193-2

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Superoxide dismutase (SOD) activity of erythrocytes from normal pregnant women (controls) and from pre-eclamptic toxaemia (PET) cases was determined by the method of Winterbourn et al. (J Lab Clin Med 1975;85:337-341) with slight modifications. Instead of the Michaelis-Menten type of plot that is usually obtained and which these authors have found to be imprecise, a linear plot was obtained when the reciprocal values for the percentage inhibition of nitroblue tetrazolium (NET) reduction were plotted against the corresponding volumes of red blood cell (RBC) extract. This plot was found to give precise values for the volumes of RBC extracts required for 50% inhibition of NET reduction. Consequently, a clearer understanding is beginning to emerge regarding the SOD activity of PET cases compared with that of normal pregnant women.

引用

页码：1 / 6

页数：6

共 15 条

[1]

Armitage P., 1971, STAT METHODS MED RES

[2] OXYGEN FREE-RADICALS AND HUMAN-DISEASES [J].

ARUOMA, OI ;

KAUR, H ;

HALLIWELL, B .

JOURNAL OF THE ROYAL SOCIETY OF HEALTH, 1991, 111 (05) :172-177

[3] SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS [J].

BEAUCHAM.C ;

FRIDOVIC.I .

ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) :276-&

[4] ANNOTATION - ACTIVATED OXYGEN AND HEMOLYSIS [J].

CARRELL, RW ;

WINTERBOURN, CC ;

RACHMILEWITZ, EA .

BRITISH JOURNAL OF HAEMATOLOGY, 1975, 30 (03) :259-264

[5]

CARRICO RJ, 1969, J BIOL CHEM, V244, P6087

[6]

HALLIWELL B, 1992, J LAB CLIN MED, V119, P598

[7]

HILL AB, 1977, SHORT TXB MED STAT, P127

[8]

ILOUNO LE, 1983, THESIS U CALABAR CAL

[9]

MIRAMI M, 1979, CLIN CHIM ACTA, V92, P337

[10]

MOSS DW, 1976, FUNDAMENTALS CLIN CH

← 1 2 →