Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum

被引：40

作者：

Okamura, Y

Takeyama, H

Sekine, T

Sakaguchi, T

Wahyudi, AT

Sato, R

Kamiya, S

Matsunaga, T ^{[1
]}

机构：

[1] Univ Tokyo, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

[2] TDK Akita Lab Corp, Akita, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 07期

关键词：

D O I：

10.1128/AEM.69.7.4274-4277.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.

引用

页码：4274 / 4277

页数：4

共 24 条

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