Production and separation of exo- and endoinulinase from Aspergillus ficuum

被引：35

作者：

Jing, W ^{[1
]}

Jin, ZY ^{[1
]}

Bo, J ^{[1
]}

Augustine, A ^{[1
]}

机构：

[1] So Yangtze Univ, Sch Food Sci & Technol, Wuxi 214036, JiangSuProvince, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2003年 / 39卷 / 01期

关键词：

Aspergillus ficuum; inulinase; exoinulinase; endoinulinase; native-PAGE; production; separation;

D O I：

10.1016/S0032-9592(02)00264-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The production of both exo- and endoinulinase by Aspergillus ficuum JNSP5-06 was investigated. Optimum fermentation conditions were found to be: inulin, 2%; yeast extract, 2%; (NH4)H2PO4, 0.5%; NaCl, 0.5%; MgSO4.7H(2)O, 0.05%; ZnSO4.7H(2)O, 0.01%; initial pH 6.5. A new and convenient method was developed to separate the inulinases by native-polyacrylamide gel electrophoresis (PAGE). Eight protein bands were obtained. Three bands were identified as exoinulinase and two bands were endoinulinase using TLC and HPLC. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：5 / 11

页数：7

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