Inhibition of the double-stranded RNA-dependent protein kinase PKR by mammalian ribosomes

被引：19

作者：

Raine, DA ^{[1
]}

Jeffrey, IW ^{[1
]}

Clemens, MJ ^{[1
]}

机构：

[1] St George Hosp, Sch Med, Dept Biochem, Cellular & Mol Sci Grp, London SW17 0RE, England

来源：

FEBS LETTERS | 1998年 / 436卷 / 03期

基金：

英国惠康基金;

关键词：

protein kinase PKR; initiation factor eIF2; ribosome; double-stranded RNA; translational control;

D O I：

10.1016/S0014-5793(98)01163-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous evidence has shown that the majority of the interferon-inducible, double-stranded RNA-dependent protein kinase PKR is associated with ribosomes in vivo. Here we show that ribosomes are inhibitory for PKR activity since they compete with dsRNA for binding to PKR, inhibit the activation of the protein kinase by dsRNA, and prevent the phosphorylation of the PKR substrate eIF2 alpha. We suggest that ribosormes constitute a reservoir of inactive PKR and that the protein kinase must be displaced from the ribosome by dsRNA in order to become activated. (C) 1998 Federation of European Biochemical Societies.

引用

页码：343 / 348

页数：6

共 46 条

[1]

BARBER GN, 1995, MOL CELL BIOL, V15, P3138

[2] THE 58-KILODALTON INHIBITOR OF THE INTERFERON-INDUCED DOUBLE-STRANDED RNA-ACTIVATED PROTEIN-KINASE IS A TETRATRICOPEPTIDE REPEAT PROTEIN WITH ONCOGENIC PROPERTIES [J].

BARBER, GN ;

THOMPSON, S ;

LEE, TG ;

STROM, T ;

JAGUS, R ;

DARVEAU, A ;

KATZE, MG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (10) :4278-4282

[3] Oncogenic potential of TAR RNA binding protein TRBP and its regulatory interaction with RNA-dependent protein kinase PKR [J].

Benkirane, M ;

Neuveut, C ;

Chun, RF ;

Smith, SM ;

Samuel, CE ;

Gatignol, A ;

Jeang, KT .

EMBO JOURNAL, 1997, 16 (03) :611-624

[4] MECHANISM OF INTERFERON ACTION - ACTIVATION OF THE HUMAN P1/ELF-2-ALPHA-PROTEIN KINASE BY INDIVIDUAL REOVIRUS S-CLASS MESSENGER-RNAS - S1 MESSENGER-RNA IS A POTENT ACTIVATOR RELATIVE TO S4 MESSENGER-RNA [J].

BISCHOFF, JR ;

SAMUEL, CE .

VIROLOGY, 1989, 172 (01) :106-115

[5] The double-stranded RNA-dependent protein kinase PKR: Structure and function [J].

Clemens, MJ ;

Elia, A .

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 1997, 17 (09) :503-524

[6]

Clemens MJ, 1996, TRANSLATIONAL CONTRO, P139

[7] A double-stranded RNA-activated protein kinase-dependent pathway mediating stress-induced apoptosis [J].

Der, SD ;

Yang, YL ;

Weissmann, C ;

Williams, BRG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (07) :3279-3283

[8] Regulation of the double-stranded RNA-dependent protein kinase PKR by RNAs encoded by a repeated sequence in the Epstein-Barr virus genome [J].

Elia, A ;

Laing, KG ;

Schofield, A ;

Tilleray, VJ ;

Clemens, MJ .

NUCLEIC ACIDS RESEARCH, 1996, 24 (22) :4471-4478

[9] THE BINDING OF DOUBLE-STRANDED-RNA AND ADENOVIRUS VAI RNA TO THE INTERFERON-INDUCED PROTEIN-KINASE [J].

GALABRU, J ;

KATZE, MG ;

ROBERT, N ;

HOVANESSIAN, AG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 178 (03) :581-589

[10]

GALABRU J, 1987, J BIOL CHEM, V262, P15538

← 1 2 3 4 5 →