The pro region of Toxoplasma ROP1 is a rhoptry-targeting signal

被引:43
作者
Bradley, PJ [1 ]
Boothroyd, JC [1 ]
机构
[1] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA
关键词
toxoplasma gondii; ROP1; rhoptry; protein targeting;
D O I
10.1016/S0020-7519(01)00242-9
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The rhoptries of Toxoplasma gondii are regulated secretory organelles involved in the invasion of host cells. Rhoptry proteins are synthesised as pre-pro-proteins that are processed first to pro-proteins upon entrance into the secretory pathway, then processed again to their mature forms late in the secretory pathway. The pro-mature processing site of the rhoptry protein ROP1 has been determined, paving the way for understanding the role of the pro region in rhoptry protein function. We demonstrate here that the ROPI pro region is sufficient for targeting a reporter protein (amino acids 34-471 of the Trypanosoma brucei VSG 117 protein) to the rhoptries. These results, together with our previous work showing that rhoptry targeting is unaffected by deletion of the pro region, indicate that the ROP I protein contains at least two signals that can function in rhoptry targeting. (C) 2001 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1177 / 1186
页数:10
相关论文
共 40 条
[1]  
Baldini A, 2000, ZEI STUD EU ECON LAW, V2, P19
[2]  
BANGS JD, 1993, J CELL SCI, V105, P1101
[3]   A soluble secretory reporter system in Trypanosoma brucei - Studies on endoplasmic reticulum targeting [J].
Bangs, JD ;
Brouch, EM ;
Ransom, DM ;
Roggy, JL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (31) :18387-18393
[4]   Restriction enzyme-mediated integration elevates transformation frequency and enables co-transfection of Taxoplasma gondii [J].
Black, M ;
Seeber, F ;
Soldati, D ;
Kim, K ;
Boothroyd, JC .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1995, 74 (01) :55-63
[5]   Identification of the pro-mature processing site of Toxoplasma ROP1 by mass spectrometry [J].
Bradley, PJ ;
Boothroyd, JC .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1999, 100 (01) :103-109
[6]  
Carruthers VB, 1997, EUR J CELL BIOL, V73, P114
[7]   PLASMODIUM-FALCIPARUM - 2 ANTIGENS OF SIMILAR SIZE ARE LOCATED IN DIFFERENT COMPARTMENTS OF THE RHOPTRY [J].
CREWTHER, PE ;
CULVENOR, JG ;
SILVA, A ;
COOPER, JA ;
ANDERS, RF .
EXPERIMENTAL PARASITOLOGY, 1990, 70 (02) :193-206
[8]   Participation of myosin in gliding motility and host cell invasion by Toxoplasma gondii [J].
Dobrowolski, JM ;
Carruthers, VB ;
Sibley, LD .
MOLECULAR MICROBIOLOGY, 1997, 26 (01) :163-173
[9]   Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite [J].
Dobrowolski, JM ;
Sibley, LD .
CELL, 1996, 84 (06) :933-939
[10]   Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene - Use as a selectable marker for stable transformation [J].
Donald, RGK ;
Carter, D ;
Ullman, B ;
Roos, DS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (24) :14010-14019