Targeted mutagenesis by duplication insertion in the radioresistant bacterium Deinococcus radiodurans:: Radiation sensitivities of catalase (katA) and superoxide dismutase (sodA) mutants

被引：113

作者：

Markillie, LM ^{[1
]}

Varnum, SM ^{[1
]}

Hradecky, P ^{[1
]}

Wong, KK ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Richland, WA 99352 USA

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 02期

关键词：

D O I：

10.1128/JB.181.2.666-669.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA) and superoxide dismutase (sodA) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.

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页码：666 / 669

页数：4

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